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Dual-Color Confocal Microscopy Illustrates Donor Origin of Endothelial Cells

Visualization of donor origin of endothelial cells in transplanted hearts using dual-color confocal microscopy at 8 weeks post-transplantation. Detection of infiltrating cells and co-localization of donor MHC class I with EC highlighted.

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Dual-Color Confocal Microscopy Illustrates Donor Origin of Endothelial Cells

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  1. B C A Recipient class I CD31 merge F D E merge Donor class I CD31 Supplemental Digital Content 1- Figure 1. Dual colour confocal microscopy illustrates donor origin of EC, 8 weeks after transplantation. PVG.RT1u hearts, 8 weeks after allotransplantation, stained with MN4-91-6 (green, against RT1Aa, recipient MHC class I) detects infiltrating cells (A), stained for CD31 (red) to detect EC (B), merging A and B shows no co-localisation. Panel D was stained with U9F4 (green, against RT1Au, donor MHC class I), or with MoAb to CD31 to detect EC (E), merging shows co-localisation, yellow, of donor MHC class I with CD31 (F).

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