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Analysis of Rabbit Serum Proteins via Coomassie Blue Staining

This study investigates the protein composition of rabbit serum, focusing on key proteins such as RMCP-1, RMCP-5, and CP-A, utilizing Coomassie Blue stain for visual detection. The analysis identifies major protein bands at molecular weights of approximately 62 kDa, 48 kDa, 33 kDa, and 25 kDa, revealing their relative abundance and characteristics under different conditions. The experiment also measures the impact of substances like 48/80, providing insights into the serum's biochemical response. The evaluation is conducted over periods of 20 minutes to 8 hours to observe protein stability and migration.

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Analysis of Rabbit Serum Proteins via Coomassie Blue Staining

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  1. Normal Rabbit Serum A Coomasie Blue Stain CP-A RMCP-1 RMCP-5 Nippo Ag Nippo Ag Nippo Ag Nippo Ag Nippo Ag tc-LIG tc-LIG tc-LIG tc-LIG tc-LIG SPON SPON SPON SPON SPON 62 kDa 48 kDa 33 kDa 25 kDa B RMCP-1 RMCP-5 CP-A 20 min 8 h 20 min 8 h 20 min 8 h tc-LIG tc-LIG tc-LIG tc-LIG tc-LIG tc-LIG SPON SPON SPON SPON SPON SPON C tc-LIG (mM) C 48/80 0.01 0.05 0.5 0.1 10 1 CP-A (~41 kDa) 48 kDa (~35 kDa) RMCP-5 33 kDa (~31 kDa) RMCP-1 25 kDa

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