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Analysis of Membrane and Cytosolic Proteins in U937 Cells: Molecular Weight and Phosphorylation States

This study investigates the molecular weights and localization of key proteins within U937 cells. We analyze proteins such as GAPDH (37 kDa) and clathrin (190 kDa), and assess the phosphorylation status of VCP at 97 kDa in the membrane, cytosol, and nucleus. Techniques like Coomassie staining are deployed to visualize protein bands corresponding to various molecular weights, including 62 kDa and 67 kDa. By comparing normalized VCP levels across different cellular compartments, we gain insights into the molecular dynamics and regulatory mechanisms in these cells.

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Analysis of Membrane and Cytosolic Proteins in U937 Cells: Molecular Weight and Phosphorylation States

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  1. membrane nucleus approx . cytosol molecular weight 37 kDa GAPDH clathrin 190 kDa 62 kDa nup62 U937 3d 7d 11d 14d 18d Retro approx . molecular weight 97 kDa VCP normalized VCP 97 kDa P - tyr membrane normalized P-tyr Coomassie 67 kDa VCP 97 kDa normalized VCP 97 kDa P - tyr cytosol normalized P-tyr Coomassie 90 kDa 97 kDa VCP normalized VCP 97 kDa P - tyr nucleus normalized P-tyr Coomassie 17 kDa A B

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