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Sysmex XE-2100

Sysmex XE-2100. NRBC Detection. NRBC Counts - Methodology. From semiconductor laser flow cytometry. STROMATOLYSER-NR ( diluent ) STROMATOLYSER-NR dye ( stain ) P assed through the laser flow cytometer for analysis. Counted using side fluorescence and forward light scatter.

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Sysmex XE-2100

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  1. Sysmex XE-2100 NRBC Detection

  2. NRBC Counts - Methodology • From semiconductor laser flow cytometry. • STROMATOLYSER-NR (diluent) • STROMATOLYSER-NR dye (stain) • Passed through the laser flow cytometer for analysis. • Counted using side fluorescence and forward light scatter.

  3. Flow Cytometry • Forward light scatter - cell size • Side fluorescence - RNA/DNA information (nucleus)

  4. NRBC Channel Explained

  5. Therefore the NRBC Scattergram…

  6. Resolution of NRBC and WBC • Clear & reliable separation of NRBC and WBC • Wbc take upp more dye than NRBC - so higher fluorescence • Smaller volume of the NRBC nucleus gives reduced forward light scatter.

  7. CORRECTING WBC COUNT

  8. The NRBC Count Algorithm NRBC NRBC% = NRBC# x 100 NRBC# + WBC# (NRBC/ 100 WBC) WBC# NRBC#

  9. Eo Ly Ba NRBC Mo Ne The Corrected WBC Count WBC NRBC

  10. NRBC # Revised Algorithm WBC region in WBC/Baso and Diff scattergram include NRBC if NRBC appear

  11. WBC & DIFF channels: Correction for NRBC’s Correct WBC Minus Correct WBC Differential Minus

  12. NRBC FlagsSwiching Algorithm NRBC-CH is measured “NRBC present” flag is monitored (WBC abnormal flag category) Detection from “NRBC%” NRBC-CH is not measured “NRBC?” flag is monitored (WBC suspect flag category) Detection from DIFF scattergram

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