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семинары “molecular biology of the cell”

семинары “molecular biology of the cell”. Федор Северин , корпус А, комн. 220 тел. 939-31-07 8-915-120-7955 E-mail: severin@belozersky.msu.ru Святослав Соколов, Дмитрий Кнорре http://yeastmolbiol.belozersky.msu.ru. - why what you are studying is interesting/important.

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семинары “molecular biology of the cell”

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  1. семинары “molecular biology of the cell” Федор Северин, корпус А, комн. 220 тел. 939-31-07 8-915-120-7955 E-mail: severin@belozersky.msu.ru Святослав Соколов, Дмитрий Кнорре http://yeastmolbiol.belozersky.msu.ru

  2. - why what you are studying is interesting/important

  3. - why what you are studying is interesting/important - what is already known

  4. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions

  5. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • - motivation of a particular experiment

  6. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup

  7. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment

  8. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions

  9. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions

  10. - why what you are studying is interesting/important • what is already known • - motivation of your project: unanswered questions • motivation of a particular experiment • general experimental setup • specific experiment • conclusions • General conclusions, models, etc.

  11. motivation of a particular experiment • general experimental setup • specific experiment • Conclusions • Scc1’s cleavage and dissociation from chromosomes • depends both in vitro and in vivo on a protein called • Esp1. Previous studies have left unanswered whether separin • is itself the protease that cleaves Scc1 • To establish whether Scc1 can serve as a cleavage sub • strate whwe purified Scc1 from insect cells exp • pressing a Flag-epitope-tagged yeast SCC1 gene from • a recombinant baculovirus. • all Scc1-P was cleaved by extracts containing • intermediate levels of Esp1 separin (Figure 2B) • Thus, Esp1 separin might indeed be the protease that cleaves Scc1. However, we cannot elimiinnate at this stage the possibility that the Scc1 cleavage • activity is due to a substoichiometric protease that copurifies with separin. Uhlmann et al., Cell, 2000

  12. Актуальность предмета исследования Что известно

  13. Актуальность предмета исследования Что известно Мотивация исследования: вопросы

  14. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента

  15. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента

  16. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента

  17. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента Выводы по результату эксперимента

  18. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента Выводы по результату эксперимента

  19. Актуальность предмета исследования Что известно Мотивация исследования: вопросы Мотивация эксперимента Общий дизайн эксперимента Детальное описание эксперимента Выводы по результату эксперимента Общие выводы, модели, гипотезы

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