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This study utilized ATH1 Microarray to analyze gene expression in Arabidopsis transverse sections. Total RNA was extracted, and cRNA probes were generated for hybridization. Data from wild type and rhd2-1 genotypes were compared, focusing on myb23 and cpc/try genes. Protoplasting and FACS were used to isolate cells for RNA extraction. The study provides insights into gene expression profiles in different sections of Arabidopsis.
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Dataset One Dataset Two Wild type rhd2-1 Genotype wer/myb23 cpc/try Cross section Transverse-section RHDZ Protoplasting + FACS RNA extraction Total RNA Total RNA mRNA extraction cRNA probe cRNA probe Hybridisation mRNA mRNA ATH1 Microarray ATH1 Microarray cRNA probe cRNA probe Hybridisation ATH1 Microarray ATH1 Microarray