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Gene

(A). WT.  MoDcl 1 knockout.  MoDcl 2 in  MoDcl 1 mutant. (B). MoDcl1. MoDcl2/MoDcl 1. 5’ Flank. 3’ Flank. Gene. Ladder KO Ect. Ladder KO KO Ect. Probe. BAR. 5’ Flank. 3’ Flank. HindIII. HindIII. 1429 bp. Flank out. Hyg /BAR-specific. (C).

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Gene

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  1. (A) WT  MoDcl1 knockout  MoDcl2 in  MoDcl1 mutant (B) MoDcl1 MoDcl2/MoDcl1 5’ Flank 3’ Flank Gene Ladder KO Ect Ladder KO KOEct Probe BAR 5’ Flank 3’ Flank HindIII HindIII 1429 bp Flank out Hyg/BAR-specific (C) 1 2 3 4 5 6 7 8 9 10 11 (D) 1 2 3 4 Gene-specific Probe hygR 5’ Flank 3’ Flank HindIII PstI 2833 bp

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