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Chapter 7. The Cell: Cytoskeleton

Chapter 7. The Cell: Cytoskeleton. AP Biology. 2005-2006. Cytoskeleton. Function structural support maintains shape of cell provides anchorage for organelles motility cell locomotion cilia, flagella, etc. regulation organizes structures & activities of cell. AP Biology.

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Chapter 7. The Cell: Cytoskeleton

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  1. Chapter 7.The Cell: Cytoskeleton AP Biology 2005-2006

  2. Cytoskeleton • Function • structural support • maintains shape of cell • provides anchorage for organelles • motility • cell locomotion • cilia, flagella, etc. • regulation • organizes structures & activities of cell AP Biology 2005-2006

  3. Cytoskeleton • Structure • network of fibers extending throughout cytoplasm • 3 main protein fibers • microtubules • microfilaments • intermediate filaments It’s a matter of size… AP Biology 2005-2006

  4. AP Biology 2005-2006

  5. Evolutionary perspective • Proteins that make up the fibers are very similar in all living things • from bacteria to humans • tubulin (all cells) • actin (eukaryote cells) • Means that they are both ancient and essential for life AP Biology 2005-2006

  6. Microtubules • Structure • thickest fibers • hollow rods about 25nm in diameter • constructed of protein, tubulin • grow or shrink as more tubulin molecules are added or removed AP Biology 2005-2006

  7. Microtubules • Function • structural support & cell movement • move chromosomes during cell division • centrioles • tracks that guide motor proteins carrying organelles to their destination • motor proteins: myosin & dynein • motility • cilia • flagella AP Biology 2005-2006

  8. Centrioles • Cell division • in animal cells, pair of centriolesorganize microtubules guiding chromosomes in cell division AP Biology 2005-2006

  9. Cilia & flagella • Extensions of eukaryotic cytoskeleton • Cilia = numerous & short (hair-like) • Flagella = 1-2/cell & longer (whip-like) • move unicellular & small multicellular organisms by propelling water past them • cilia sweep mucus & debris from lungs • flagellum of sperm cells AP Biology 2005-2006

  10. Cilia • Oar-like movement • alternating power & recovery strokes • generate force perpendicular to cilia’s axis AP Biology 2005-2006

  11. Flagella • undulatory movement • force generated parallel to flagellum’s axis AP Biology 2005-2006

  12. Cilia & Flagella • Structure • remember 9+2! • 9 pairs of microtubules around 2 single microtubules in center • bending of cilia & flagella is driven by motor protein • dynein • requires ATP AP Biology 2005-2006

  13. AP Biology 2005-2006

  14. Microfilaments (actin filaments) • Structure • thinnest class of fibers • solid rods of protein, actin • twisted double chain of actin subunits • about 7nm in diameter • Function • 3-D network inside cell membrane • in muscle cells, actin filaments interact with myosin filaments to create muscle contraction AP Biology 2005-2006

  15. “Let’s go to the video tape!” (play movie here) Microfilaments (actin filaments) • Dynamic process • actin filaments constantly form & dissolve making the cytoplasm liquid or stiff during movement • movement of Amoeba • cytoplasmic streaming in plant cells • speeds distribution of materials AP Biology 2005-2006

  16. Intermediate filaments • Structure • specialized for bearing tension • built from keratin proteins • same protein as hair • intermediate in size 8-12nm • Function • hold “things” in place inside cell • more permanent fixtures of cytoskeleton • reinforce cell shape & fix organelle location • nucleus is held in place by a network of intermediate filaments AP Biology 2005-2006

  17. Summary • Microtubules • thickest • cell structure & cell motility • tubulin • Microfilaments • thinnest • internal movements within cell • actin, myosin • Intermediate filaments • intermediate • more permanent fixtures • keratin • actin • microtubule • nuclei AP Biology 2005-2006

  18. AP Biology 2005-2006

  19. AP Biology 2005-2006

  20. Cell Junctions • Where cells touch each other… AP Biology 2005-2006

  21. Plant cell wall • Structure • cellulose • primary cell wall • secondary cell wall • middle lamella = sticky polysaccharides AP Biology 2005-2006

  22. Intercellular junctions • Plant cells • plasmodesmata • channels allowing cytosol to pass between cells plasmodesmata AP Biology 2005-2006

  23. Animal cell surface • Extracellular matrix • collagen fibers in network of glycoproteins • support • adhesion • movement • regulation AP Biology 2005-2006

  24. Intercellular junctions in animals AP Biology 2005-2006

  25. Intercellular junctions • Animal cells • tight junctions • membranes of adjacent cells fused forming barrier between cells • forces material through cell membrane • gap junctions • communicating junctions • allow cytoplasmic movement between adjacent cells • desmosomes • anchoring junctions • fasten cells together in strong sheets AP Biology 2005-2006

  26. AP Biology 2005-2006

  27. AP Biology 2005-2006

  28. A cell is a living unit greater than the sum of its parts AP Biology 2005-2006

  29. And now for some… Stupid Penguin Tricks…. Any Questions?? AP Biology 2005-2006

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