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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

GEIL-GTLLF 2008. Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies. Part four : Lymphocytic maturation. IV.1. SSC. SSC. SSC. SSC. SSC. SSC. FLy. FLy. FLy. FLx. CD45. CD45. FLx. FSC. FLx. FLn. CD45. CD45. Lymphober. Backgating.

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Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies

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  1. GEIL-GTLLF 2008 Multiparametric flow cytometry of normal human bone marrow: analysis and display strategies Part four : Lymphocytic maturation IV.1

  2. SSC SSC SSC SSC SSC SSC FLy FLy FLy FLx CD45 CD45 FLx FSC FLx FLn CD45 CD45 Lymphober Backgating Display Strategy IV.2

  3. Informative combinations Tube 1 : CD11b/CD14/CD16/CD45 Tube 9 : CD64/CD24/CD10/CD45 CD11b/CD16 p. IV.4 CD24/CD10 p. IV.9 Tube 5 : CD38/DR/CD34/CD45 Tube 10 : CD7/CD117/CD34/CD45 CD38/CD34 p. IV.5 CD7/CD117 p. IV.10 CD7/CD34 p. IV.11 . Tube 6 : CD38/CD10/CD19/CD45 CD38/CD10 p. IV.6 Tube 11 : CD22/CD10/CD20/CD45 CD22/CD10 p. IV.12 Tube 8 : CD34/CD56/CD19/CD45 CD10/CD20 p. IV.13 CD34/CD19 p. IV.7 CD56/CD19 p. IV.8 IV.3

  4. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD16PC6 CD16PC5 CD16PC5 CD11bFITC CD11bFITC CD11bFITC CD11b/CD16 Gating CD16 Lymphober Gating CD11b IV.4

  5. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 CD38FITC CD38FITC CD38ITC CD38/CD34 Gating CD38 Gating CD34 Lymphober IV.5

  6. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD10PE CD10PE CD10PE CD38FITC CD38FITC CD38FITC CD38/CD10 Gating CD38 Gating CD10 Lymphober IV.6

  7. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD19PC6 CD19PC5 CD19PC5 CD34FITC CD34FITC CD34FITC CD34/CD19 Gating CD19 Lymphober Gating CD34 IV.7

  8. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD19PC6 CD19PC5 CD19PC5 CD56FITC CD56FITC CD56ITC CD56/CD19 Gating CD19 Lymphober Gating CD56 IV.8

  9. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD10PC5 CD10PC5 CD10PC5 CD24PE CD24PE CD24PE CD24/CD10 Gating CD10 Lymphober Gating CD24 IV.9

  10. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD117PE CD117PE CD117PE CD7FITC CD7FITC CD7FITC CD7/CD117 Gating CD117 Lymphober Gating CD7 IV.10

  11. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD34PC5 CD34PC5 CD34PC5 CD7FITC CD7FITC CD7FITC CD7/CD34 Gating CD34 Lymphober Gating CD7 IV.11

  12. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD10PE CD10PE CD10PE CD22FITC CD22FITC CD22FITC CD22/CD10 Gating CD10 Lymphober Gating CD22 IV.12

  13. SSC SSC SSC FSC CD45 CD45 FLy FLy FLy SSC-Height SSC-Height SSCHeight FLx FLx FLx CD45ECD CD45ECD CD45ECD CD20PC5 CD20PC5 CD20PC5 CD10PE CD10PE CD10PE CD10/CD20 Gating CD10 Lymphober Gating CD20 IV.13

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