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Hindbrain Neurons

Hindbrain Neurons. Question:. How will the alteration of input affect neurons in the CNS dedicated to inner ear sensory input processing? By manipulating the ear, either by removing it or by adding extra ears, we can determine the effects of afferents on their target neuron(s) in the CNS.

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Hindbrain Neurons

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  1. Hindbrain Neurons

  2. Question: • How will the alteration of input affect neurons in the CNS dedicated to inner ear sensory input processing? • By manipulating the ear, either by removing it or by adding extra ears, we can determine the effects of afferents on their target neuron(s) in the CNS

  3. Mauthner Cell • Single, large, pair of reticulospinal neurons at the level of the ear in the hindbrain of most aquatic animals • Important component of the C-start escape response 25µm

  4. Mauthner Cell • Early, but not late, ear ablation has been shown to result in the absence of the Mauthner cell • Has been suggested to be a surgical artifact • Surviving Mauthner cells show a reduction in dendritic branching • Previous studies each only focused on a single time point of ear removal • I removed the ear at various time points in development to answer the following questions:

  5. Mauthner Cell Questions • 1) Is there a critical period of a Mauthner cell during which it depends on the ear for survival? • 2) Does the stage at which the ear is removed affect the degree of dendritic branching of surviving Mauthner cells?

  6. Dendritic Branching following ear loss Control Remove and Replace Ear 23 Terminal Branches 26 Terminal Branches 19 Terminal Branches 24 Terminal Branches 25µm 24 Terminal Branches 3 Terminal Branches 32 Terminal Branches 9 Terminal Branches Ear Removed at Placode (Stage 26) Ear Removed at Late Otic Vesicle (Stage 40)

  7. Dendritic Branching with extra ear Extra Ear 21 Terminal Branches 33 Terminal Branches 50µm 50µm

  8. Control Dendritic Branching Treated * *** *** *** n = 12 n = 12 n = 12 n = 12 n = 12 n = 10 Remove Placode (24-26) Remove Early Otic Vesicle (27-30) Remove Late Otic Vesicle (27-30) Control Remove-Replace Extra Ear t-test * p < 0.05 *** p < 0.001

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