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Lysozyme Protein Crystal Growth in Microgravity

Lysozyme Protein Crystal Growth in Microgravity. Celeste Brown, Jackson Middle School; 7 th grade Josephine Smith, Jackson Middle School; 7 th grade Teacher Facilitator: Jennifer Kelley; Jackson Middle School Scientific Advisor: Dr. Eric Gouaux; Oregon Health and Science University.

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Lysozyme Protein Crystal Growth in Microgravity

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  1. Lysozyme Protein Crystal Growth in Microgravity Celeste Brown, Jackson Middle School; 7th grade Josephine Smith, Jackson Middle School; 7th grade Teacher Facilitator: Jennifer Kelley; Jackson Middle School Scientific Advisor: Dr. Eric Gouaux; Oregon Health and Science University

  2. Our idea • Wanted to help friend Brittany • Looked at cancer research projects • Chose Urokinase protein crystal growth

  3. Hypothesis We think the Lysozyme crystals will grow better and there will be more crystals in microgravity than when they are grown on Earth. We think this because gravity will affect the ionic bonds, making them weaker. In microgravity however, the bonds should be stronger, therefore giving us better results.

  4. Experimental Design • 2:1 of protein to NaCl • Lysozyme • NaCl 5% • A 3 mm by 3 mm piece of cotton in a few of the wells.

  5. Experiment Conditions Ground truth experiment • Residential home • Lab conditions Space shuttle: • Microgravity

  6. Problems/Solutions • Urokinase $$$ • New enzyme • Shuttle delay • Transportation • Diffraction lab

  7. Results • Controlled environment=crystals. • Didn’t find crystals in the space • shuttle or home ground truth samples, • because they were kept in uncontrollable • environments. • Crystals found in the lab ground truth, • because the lab has a stable • environment.

  8. Conclusion • Lab conditions need to be constant • Shipping – crystals should have been frozen • Hypothesis: Unsure due to issues with experiment conditions

  9. Acknowledgements • Dr. Eric Gouaux, Sr. Scientist, Vollum Institute, Oregon Health & Science University • Sigma- Aldrich Corporation • The Stanford Synchrotron Radiation Lightsource at SLAC National Accelerator Laboratory • Jennifer Kelley, Teacher Facilitator, Jackson Middle School • Our Parents!

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