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FSU-REAP

FSU-REAP. June 14- July 16 Jessica Bundy. Teachers at school told me about this program I soon became Dr. Lodhi’s apprentice. His project: The amount of useful DNA present on cell phones Sarah and I also helped Camille with her lab manual. Then Stefanie Phillips came and helped us also.

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FSU-REAP

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  1. FSU-REAP June 14- July 16 Jessica Bundy

  2. Teachers at school told me about this program • I soon became Dr. Lodhi’s apprentice. • His project: The amount of useful DNA present on cell phones • Sarah and I also helped Camille with her lab manual. • Then Stefanie Phillips came and helped us also.

  3. Tools of the Trade (for the lab anyway) • I have been using pipets, the vortex, centrifuges, the water bath, and the hot plate endlessly. Uses: -pipets: measure volume, transport liquids -vortex: mixes ingredients, DNA, etc. -centrifuges: spin ingredients down -water bath: heats samples for extended periods of time -hot plate: stir and heat

  4. Adventures with Camille • Camille, on the second day, gave us a “forensic biology in 5 minutes” speech • Sample ID process: Identification, Confirmation, Species Origin, Blood Grouping Test (if blood) • Labs with Camille: Luminol, Hemastix, Phenoylphthaline, O- Tolidine, and Hemident

  5. Luminol Test • During this test we cut a sample and sprayed it with the luminol fluid in the dark room. The the blood sample started to glow a neon bluish color.

  6. DNA Extraction • Extraction process • Sarah and I eventually got to extract our own DNA smaples, after watching Dr. Lodhi and taking notes. • We mostly took DNA from cheek swab samples and samples from our own cell phones.

  7. Sarah and I got to use PCI to help break the membranes and the protein layers of the cells to get the DNA.

  8. Applied Boisystems Training • Trainers: Michelle Shepard and Shanin Lodhi • We learned how to operate the Realtime PCR and the Genetic Analyzer. • Also, we learned how the kits that AB sells use ladders, dyes, and buffers to analye DNA.

  9. Also, Sarah and I spent a lot of time talking to Shanin and Michelle about biology, since we have only had high school level biology class. We learned about TAQ, inhibitors, exactly how the machines work, and even heard a few crime scene stories. DNA workflow: extraction, quantation, amplification, and analysis

  10. REAP’s influence • The REAP program helped me realize that I don’t want to work in a lab. • I want to be a NC teaching fellow for either chemistry or history. • Even though I don’t want to be a forensic biologist or a chemist, I have more knowledge about how much the lab influences the criminal justice system and how the methods and technology operate in crime labs.

  11. Thanks so much to everyone who helped me and for giving me this opportunity! I learned a lot and good luck to everyone finishing your summer research projects!

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