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Microbiology

This guide covers essential components of the compound microscope used in microbiology. Key parts include the ocular (10x), various objective lenses (scanning 5x, low power 10x, high power 45x, and oil immersion 100x), stage control lever, adjustment knobs, and an iris diaphragm lever. It explains total magnification calculations and introduces basic bacterial morphologies such as cocci, bacilli, and spiral forms. Examples of bacteria examined through these lenses include Salmonella typhosa, Streptococcus lactis, and Clostridium botulinum.

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Microbiology

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  1. Microbiology

  2. Lab # 1 The Compound Microscope

  3. Parts of the Microscope • 1. Ocular (eyepiece) 10x • 2. Objectives (Lenses) • Scanning Objective 5x • Low Power Objective 10x • High Power Objective 45x • Oil Immersion Lens 100x • 3. Stage Control Lever • 4. Adjustment Knobs • Coarse Adjustment • Fine Adjustment • 5. Iris Diaphragm Lever

  4. Total Magnification • Ocular x Objective • TM • Scanning 50x • Low Power 100x • High Power 450x • Oil Immersion Lens 1000x

  5. 3 Bacterial Morphologies • 1. Cocci • 2. Bacilli • 3. Spiral

  6. Salmonella typhosa

  7. Streptococcus lactis

  8. Proteus vulgaris

  9. Clostridium botulinum

  10. Agrobacteriumtumefaciens

  11. Rhodospirilliumrubrum

  12. Micrococcus luteus

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