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Real-Time Quantitative PCR

Real-Time Quantitative PCR. Jimmy Paden Jacci Tonn Melanie O’Callaghan. What is the Method?. Real-Time PCR delivers powerful capabilities for generating and analyzing data for a wide variety of applications. Highly sensitive quantification of transcriptional levels of the gene of interest.

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Real-Time Quantitative PCR

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  1. Real-Time Quantitative PCR Jimmy Paden Jacci Tonn Melanie O’Callaghan

  2. What is the Method? • Real-Time PCR delivers powerful capabilities for generating and analyzing data for a wide variety of applications. • Highly sensitive quantification of transcriptional levels of the gene of interest

  3. How is it performed?

  4. What do you learn from such a method? • Can find out the quantity of genes that were started with • How many cycles it takes to make a certain number of genes • The less cycles means the more genes that were present at the beginning

  5. Why do this Method? • Faster • Can do it in a few hours not days • Quantitative

  6. DATA • http://www.ashi-hla.org/publicationfiles/ASHI_Quarterly/25_3_2001/The%20Usefullness%20of%20Quantitative.pdf

  7. References • http://www.ashi-hla.org/publicationfiles/ASHI_Quarterly/25_3_2001/The%20Usefullness%20of%20Quantitative.pdf • www.bio.davidson.edu/Courses/genomics/method/realtimepcr.html • www.dorak.info/genetics/realtime.html • www.dna.iastate.edu/frame_qpcr.html

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