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Pumps and Carriers

Pumps and Carriers. Peter Maloney pmaloney@jhmi.edu. • Chemiosmotic Circuits • Rules of Thumb • Classification • Experiments in Glucose Transport • Structure and Mechanism • The Na Circuit of Mammalian Cells. Pumps and Carriers. Peter Maloney pmaloney@jhmi.edu.

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Pumps and Carriers

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  1. Pumps and Carriers Peter Maloney pmaloney@jhmi.edu

  2. •Chemiosmotic Circuits• Rules of Thumb• Classification• Experiments in Glucose Transport• Structure and Mechanism• The Na Circuit of Mammalian Cells Pumps and Carriers Peter Maloney pmaloney@jhmi.edu

  3. A Simple Chemiosmotic Cycle

  4. Superfamilies of ATP-Driven Pumps

  5. •Chemiosmotic Circuits•Rules of Thumb• Classification• Experiments in Glucose Transport• Structure and Mechanism• The Na Circuit of Mammalian Cells Pumps and Carriers Peter Maloney pmaloney@jhmi.edu

  6. 0.2 µ The Nernst Equation EK = -(RT/zF) ln [IN] / [OUT] EK = -120 mV (912 of 377,000 K+ leave)

  7. Fractional Loss in Cells of Different Radii

  8. Rules of Thumb • Movement of only a few charges will generate a membrane potential of significant size (± 120 mV) • Internal and external concentrations do not change • If a chemical gradient forms, think carefully

  9. Acidification of the Lysosome

  10. Acidification of the Lysosome

  11. •Chemiosmotic Circuits• Rules of Thumb•Classification• Experiments in Glucose Transport• Structure and Mechanism• The Na Circuit of Mammalian Cells Pumps and Carriers Peter Maloney pmaloney@jhmi.edu

  12. Classification of Transporters http://www.biology.ucsd.edu/~msaier/transport/

  13. GeneralNomenclature Carrier = Facilitator = Porter Common Biochemical Mechanisms Uniport (GLUT1*) = Facilitated Diffusion Antiport (OxlT*) = Exchange or Counter-transport Symport (LacY*) = Cotransport * Members of the Major Facilitator Superfamily

  14. •Chemiosmotic Circuits• Rules of Thumb• Classification•Experiments in Glucose Transport• Structure and Mechanism• The Na Circuit of Mammalian Cells Pumps and Carriers Peter Maloney pmaloney@jhmi.edu

  15. Experiments with the Red Cell

  16. The Carrier Hypothesis

  17. Variants of the Carrier Model ’ ’

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