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Comprehensive Urine Sample Handling and Processing Protocol

This document outlines a detailed protocol for the handling and processing of urine samples, including freezing, thawing, and spinning techniques. The steps include freezing specimens at -20ºC, processing through thawing and spinning, and pooling individual samples to create four identical samples. The protocol also includes statistical analysis with Tukey's HSD, highlighting significant differences between various processing methods. Key performance metrics and statistical results are provided for efficacy review.

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Comprehensive Urine Sample Handling and Processing Protocol

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  1. Urine sample handling 1 Freeze @ -20ºC Process Spin Thaw 2 Freeze @ -20ºC Process Thaw Spin Pool individual urine samples 3 Freeze @ -20ºC Process Spin Thaw Split into 4 identical samples RT 4 hrs 4 Freeze @ -20ºC Process Thaw Spin

  2. 12 10 8 6 4 2 0 0.0 0.2 0.4 0.6 0.8 1.0 Total # of feature: 15030 S/F/T vs. F/T/S RT/S/F/T vs. RT/F/T/S Density 0.0 0.2 0.4 0.6 0.8 1.0 Tukey HSD P value between sub groups

  3. S/F/T vs. F/T/S RT/S/F/T vs. RT/F/T/S P (Two-Tailed) = 0.013 -3 < t < 3 t < -3 t > 3 t < -3 -3 < t < 3 t > 3 122 14825 83 196 14762 72 4 Randomize Feature set 20 X MW/10000 2 0 3 2 # X 100 1 0

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