1 / 13

p16INK4A expression and HPV-L1 detection in the uterine cervix:

p16INK4A expression and HPV-L1 detection in the uterine cervix: cyto-histological and immunoistochemical study M.A. Caponio, T. Addati, G. Giannone, *G. Falco, A. Labriola, S. Petroni, V. Rubini, G. Simone UOC ANATOMIA PATOLOGICA *UOC GINECOLOGIA IRCCS – Istituto Tumori “Giovanni Paolo II”.

garin
Télécharger la présentation

p16INK4A expression and HPV-L1 detection in the uterine cervix:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. p16INK4A expression and HPV-L1 detection in the uterine cervix: cyto-histological and immunoistochemical study M.A. Caponio, T. Addati, G. Giannone, *G. Falco, A. Labriola, S. Petroni, V. Rubini, G. Simone UOC ANATOMIA PATOLOGICA *UOC GINECOLOGIA IRCCS – Istituto Tumori “Giovanni Paolo II”

  2. OBJECT: The most important event in carcinogenesis of the cervical cancer (CC) is the infection by HPV and the viral integration in cellular DNA. The “progression timing“ between the developement of the lesion in CC after viral integration is unknown. P16INK4A protein is tightly correlated to expression of HPV-L1 protein.

  3. Mechanism of p16INK4a over-expression

  4. METHODS 137 women underwent Pap Test using LBC. 78 cases had the corresponding bioptic/surgical sample. 40 cases were analised for HPV-L1 protein expression both in LBC and in the corresponding tissue sample. AIM The prevalence of the lesions, the cyto-histological correlation, the presence of HPV and the incidence of p16 (score 0 to 4), were observed.

  5. RESULTS (1) In none of the 35 evaluated cases with Negative cytology, (including 6 HPV+) and in 18 ASCUS, p16 immunoreactivity (IR) has been evidenced. IR has been found in 1/4 cases AGUS, in 4/20 L-SIL, in 6/11 H-SIL and in 2/4 SCC.

  6. RESULTS (2) In 51/78 cases, in which the histological sample was available, the p16 IR has been determined: a strong IR was present in 1/28 negative cases, 2/8 lesions CIN1, 9/12 CIN2-3, 3/3 SCC. In the evaluable cases (excluding ASCUS, AGUS and L-SIL) the Overall Agreement was 91% (83.3% with LSIL).

  7. RESULTS (3) In 18 evaluable cases, p16 has been determined both on the cytological and histological samples: in 2 cases* IR was present only on the cytological sample, while in 1 case** the opposite event was observed.

  8. RESULTS (4) In 75 cases the presence of HPV has been determined: 15 case p16 IR positive also presented infection by LR-HR HPV; in 21/44 cases that didn't express p16 IR, LR-HR HPV infection was detected.

  9. In 40 cases, analised for HPV-L1 expression, we observed the different localization in 1 case L1+/P16+ in HSIL. HPV-L1 P 16

  10. 1 case L1+/P16+ in HSIL Cyto-histological HPV-L1 detection HPV-L1 HPV-L1

  11. P 16 P16 detection in basal layer P 16

  12. Cytologycal L1+/P16- in H-SIL HPV-L1 P 16

  13. CONCLUSIONS: Our study shows that the presence of p16 and HPV-L1 protein in cyto-histological samples could be useful in giving information on viral integration and lesions progression.

More Related