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RT-PCR PowerPoint Presentation

RT-PCR

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RT-PCR

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Presentation Transcript

  1. RT-PCR Detection of Specific RNAs

  2. Annealing of Downstream Primer to RNA

  3. Reverse Transcription With AMV Reverse Transcriptase

  4. RNA Copied From 3’ to 5’ into cDNA

  5. Amplification of cDNA by PCR