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Comparative Analysis of Colony-Forming Efficiency with Protein Mutations

This study examines the impact of genetic mutations on colony-forming frequency and clonogenic efficiency. The experiments focus on different mutations and their effects on FAK and Src signaling pathways.

hyun-chong
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Comparative Analysis of Colony-Forming Efficiency with Protein Mutations

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  1. * 35 vector PaxY118F cl.1 30 PaxY118F cl.2 25 20 15 10 5 0 100 80 60 40 20 0 A v-Src Colony-forming frequency (%) FAK+/+ FAK -/- GFP-PaxY118F: -- cl. 1 -- cl. 1 cl. 2 FAK+/+ [Src] FAK-/- [Src] Ab: GFP PaxpoY118 GAPDH Clonogenic Efficiency (%) v-Src FAK+/+ [Src] FAK-/- [Src] FAK+/+ FAK -/- 35 mock GFP-WT-Pax:- + - + 30 GFP-WT-Pax 25 Colony-forming frequency (%) 20 Ab: GFP 15 10 GAPDH 5 0 FAK+/+[v-Src] FAK-/-[v-Src] v-Src B 30 vector FAK+/+ FAK -/- 25 p120Y228F 20 Flag-p120Y228F : -- + -- + Colony-forming frequency (%) 15 Ab: FLAG p120poY228 GAPDH 10 5 0 FAK+/+ [Src] FAK-/- [Src] C v-Src 35 FAK+/+ FAK -/- 30 vector GST-ShcY239/240F: -- + -- + ShcY239/240F 25 Colony-forming frequency (%) 20 Ab: GST ShcpoY239/240 GAPDH 15 10 5 0 FAK+/+ [Src] FAK-/- [Src]

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