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Amino Acids

Amino Acids. C483 Spring 2013. Questions. 1. Amino acids with non- ionizable side chains are zwitterions when they are ________. A ) in any solution D) in alkaline solutions only B ) at physiological pH, pH = 7.4 E) All of the above C ) in acidic solutions only

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Amino Acids

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  1. Amino Acids C483 Spring 2013

  2. Questions 1. Amino acids with non-ionizable side chains are zwitterions when they are ________. A) in any solution D) in alkaline solutions only B) at physiological pH, pH = 7.4 E) All of the above C) in acidic solutions only 2. Tyrosine and tryptophan are less hydrophobic than phenylalanine because A) phenylalanine is a phenol. B) phenylalanine has no polar group in the side chain. C) phenylalanine has an indole group. D) tyrosine and tryptophan have smaller R groups. E) All of the above. 3. ) Basic amino acids are ________ (positive, negative) at pH 7 and acidic R group amino acids are ________ (positive, negative) at pH 7. A) negative; positive C) positive; negative B) negative; negative D) positive; positive

  3. 4. A polypeptide chain may have abrupt changes in direction and restriction in geometry because of the presence of A) arginine. B) glycine. C) proline. D) leucine or isoleucine. 5. The pKa's of arginine's α-Carboxyl group, α-Amino group and side chain are 1.8, 9.0 and 12.5, respectively. Calculate the isoelectric point. A) 7.8 B) 7.2 C) 10.8 D) 5.4 6. What is the net charge on the dipeptide Arg-Pro at pH 9.0? The table below gives the pKa's of the ionizable groups on the free amino acids. A) -1 B) -0.5 C) 0 D) +0.5 E) +1

  4. Amino Acid Structure • Alpha carbon • Sidechain • Proteins • peptides

  5. Stereochemisty • L-amino acids • Glycine • R/S vs D/L • L-isoleucine • racemization

  6. Common Amino Acids • 20 Common Amino acids • Groups • Aliphatic • Aromatic • Sulfur • Hydroxyl • Positively charged • Negatively charged • Hydrophobicity

  7. Aliphatic • Sidechainstereochem • Secondary amine • “Highly” hydrophobic in blue

  8. Aromatic

  9. Sulfur Containing • Disulfide bonds

  10. Hydroxyl/Amide Containing • Stereochem • H-bonding • Highly hydrophilic in red

  11. Charged

  12. Hydrophobicity • Hydrophobic effect • Relative scales

  13. Other Amino Acids • The “other three” • Modified Amino acids • Oxidation • Phosphorylation • amination

  14. Amino Acid Derivatives • From which amino acids are these molecules derived?

  15. Ionization of Amino Acids • Polyprotic acids • Alanine • pKa1 = 2.4 • pKa2 = 9.9 • Zwitterion • More acidic than typical carboxylic acid

  16. Titration Curve

  17. Titration Curve Which amino acid?

  18. Ionization States Of Amino Acids G F E D C B A

  19. Answers • B • B • C • C • C • D

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