Figure 7

1. Figure 7 Suppression of S.L. mutants in with x x x x x x x x x x x Gal80 x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x x h1894 y1966 5'SS X-link y1970 h1898 x x Genetic map Physical map * Interacting proteins Conservation Prp8p prp28-1 2nd step prp17 prp17 prp28 U4-cs1 U6-UA sky1 prp22 brr2-1 prp22 3’SS 5’SS BP U6 0 100% Lab.Ref  Other features of mutant Mutant name and amino acid change -NH2 Y2H Y2H Y2H Y2H x prp8-cat (a) prp8-cat (a) prp8-cat (a) prp8-cat (a) prp8-cat (b) prp8-cat (b) prp8-cat (b) prp8-cat (b) prp8-cat (b) prp8-cat (b) prp8-cat (b) prp8-cat (b) prp8-cat (c) prp8-cat (c) prp8-cat (c) prp8-cat (c) prp8-cat (c) prp8-cat (c) prp8-5 prp8-B141 prp8-B131 prp8-B132 prp8-B142 prp8-161 prp8-2 prp8-cat (d) prp8-cat (d) prp8-cat (d) prp8-cat (d) prp8-cat (d) prp8-cat (d) prp8-cat (d) prp8-cat (d) prp8-C124 prp8-39 dbf3-1 prp8-C133 prp8-C134 prp8-syf77 prp8-scf103E prp8-scf110F prp8- scf103C prp8-syf14 prp8-syf102 prp8-8 (C) prp8-C125 prp8-scf103A prp8-C122 prp8-C123 prp8-C121 prp8-cat (e) prp8-cat (e) prp8-cat (e) prp8-cat (e) prp8-cat (e) prp8-cat (e) shm1-1 prp8-cat (e) prp8-cat (e) prp8-D135 prp8-160 prp8-501 prp8-D103 prp8-D104 prp8-D105 prp8-D106/7 prp8-cat (e) prp8-cat (e) prp8-cat (e) prp8-201 (e) prp8-cat (e) prp8-cat (e) prp8-D143 prp8-156 prp8-157 prp8-151/D136 prp8-152 prp8-162 prp8-cat (e) prp8-101 prp8-D102 prp8-152b prp8-153 prp8-154 prp8-155 prp8-52 prp8-28 spp42-1 prp8-D144 prp8-1 prp8-rp prp8-21 prp8-9 R236G L261P L280P E362D K611R E624G N643S V644A D651G D651N H659P K684E E788G E788V N796S W856R E860K Q861R I898K I857T, Y923C, E942G Y923C, E942G 7 different mutations 5 different mutations P986T P1015S L1066P N1067K N1087H D1094A D1094N/V M1095T, I1104M V1098D/I N1099K, R1105L L1107T P1191L/S/T D1192Y N1194D M1399I E1450K P1489L I1444V, T1565A, V1621A Q1455P, D1485G, H1592R L1557F unknown unknown K1563I unknown unknown G1564R T1565A, N1721Y, V1752A W1575C, E1576K, G1636S W1575R E1576V W1609R, N1618D L1624M, L1634F L1641F T1685I P1688L P1688R D1700E S1745P, D1778G R1753K/A/Q/E A1754V L1755A I1775T, D1778E D1778R, L1779M, G1635D L1794F K1807R, S1829C, E1832A N1809D E1817G L1823P S1829C, E1832V I1825K, L1835F E1832A, L1891P F1834L, V1946A F1834S, R1922G F1834L, F1880S F1834S/L F1851L V1860D V1860N T1861P V1862A/Y V1862D K1864E N1869D, T1982A 4 different mutations N1869D N1869D, S1970R V1870N I1875T N1883D E1960K (psf1-1) E1960G S1970R T1982A T1982A, SA1966/7AG T1982A, V1987A Y2037H, I2051T I2259N G2298D F2176S, Q2313R, T2364A G2347D Retinitis Pigmentosa (fig.3D) (slt21), not known not known slow growth ts, ts, ts Cell cycle defect, ts cs ts, suppresses cs prp22-S635A S.L. with prp22-S635A cs ts ts, S.L. with U4 G14C Rescues ts prp16-101 (R686I) ts, S.L. with prp16-301 Increased binding to Brr2 ts, Decreased binding () to Brr2 S.pombe equivalent of G2248D ts, S.L. with slt22-1, U5 ,  Brr2 Retinitis Pigmentosa ts with U2 mutation DB.1, 3, TC.P DB.1 DB.1, 3, TC.P DB.1, 3 DB.1 DB.1, 3 DB.1 DB.1 DB.1, 3 DB.1 DB.1, 3 DB.1 DB.1,3, TC.P DB.1 DB.1 DB.1 DB.1 DB.1 JB.U, 16 CG.4 CG.4 CG.4 CG.4 MK.19 JB.U, 16 DB.3 DB.3 DB.3 DB.1, 3 TC.P DB.1 DB.1 DB.1, 3 DB.1 DB.3 DB.1 DB.1, 3 DB.1, 3 CG.4, 5 XF.6 LJ.7 CG.4, 6 CG.4, 6 MKu.8 MKu.8 MKu.8 MKu.8 MKu.8 MKu.8 CG.9 CG.4, 5 MKu.8 CG.4, 5, 8, TC.P CG.4, 5 CG.4, 5, 6, TC.P DB.1 DB.1, 3 DB.1 DB.1 DB.1, 3 DB.1 MK, TC.P MK, TC.P BS.21 DB.1 BS.21 MK, TC.P MK, TC.P MK, TC.P MK, TC.P DB.1 CG.4 MK.19 MK, TC.P TC.P MK, TC.P CG.5, 4 CG.5 CG.5 CG.5 DB.1 DB.1, 3 DB.1 DB. 1, 2, 3, TC.P DB.1 DB.1, 3 CG.4 MK.10, 19 MK.10 MK.10, 19, CG. 4 MK.10 MK.19 DB.1 MK, TC.P CG.5, 17, 18 CG.5 MK.10, CG.4 MK.10 MK.10 MK.10 JB.11 JB.11 AK.U CG.4 JB.13, 11, 20 CI.12 XD.14 CG.15 -h1 y58- x Prp39 Prp40 y98 h35 Lin1/Snu40 C-Brr2 1282-1749 NLS y135 h83 x -h227 y300- Ph. D. Snu114 Y2H   Y2H Y2H -h525 y600- x Y2H x Putative coiled coil interaction Exo84  -h827 y900- Y2H h986 y1059 RRM  h1078 y1151 -h1127 y1200- Snp1 Y2H h1300 y1372 3.1 3'-SS fidelity y1546 y1547 3.2 h1588 y1660 x Pyr Pyr Pyr h1669 y1741 Pyr 5'SS holding domain IV   Y2H    Pyr h2093 y2172 Pyr h2094 y2173 x MPN Brr2    h2233 y2310 x -COOH h2335 y2413