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Differentiation of ES Cells to Insulin-Expressing Colonies Protocol

A detailed guide for generating insulin-expressing colonies from embryonic stem cells using monothioglycerol and NEA combination.

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Differentiation of ES Cells to Insulin-Expressing Colonies Protocol

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  1. Supplemental Figure 1 ES EB Stage Tertiary Culture Stage Colony Formation Serum Serum Replacement Semi-solid culture D7-3o D10-3o D2 EB D6 EB Add NEA Fluorescence based Sorting High MTG Low MTG Supplemental Figure 1. Protocol for differentiation of ES cells to insulin-expressing colonies. ES: embryonic stem cells; EB: embryoid body; MTG: monothioglycerol; NEA: a combination of nicotinamide, exendin-4 and activin B.

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