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Concentration Matters

This study emphasizes the critical importance of DNA concentration for reliable sequencing results using the 3130XL. We require 50 ng of DNA in 10 µL, running 5 µL on the sequencer. Notably, samples at 20 ng show enhanced call reliability, improved signal intensity, and better trace scores compared to lower concentrations. The Contiguous Read Length reflects the longest uninterrupted base stretch with a quality score above a set threshold. The QV20+ value quantifies the bases with quality scores ≥ 20, ensuring a 99% probability of accuracy in base calling.

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Concentration Matters

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  1. 10 ng of sample (note: we request 50 ng of DNA in 10ul and run 5ul on the 3130xl) Concentration Matters Note the areas of low resolution 20 ng of sample (note: we request 50 ng of DNA in 10ul and run 5ul on the 3130xl) The call reliability has drastically increased at 20 ng in the same region

  2. Note the increased signal intensity and trace scores for 20 ng The Contiguous Read Length is the longest uninterrupted stretch of bases with quality higher than a specific limit. QV20+ value is the total number of bases in the entire trace that have basecaller quality value greater than or equal to 20 (99% probability the call is correct).

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