1 / 1

Supplemental Figure 4

Supplemental Figure 4. a. Succinate Succinate+ADP Succinate+Oligo Succinate+FCCP. b. Succinate Succinate+ADP Succinate+Oligo Succinate+FCCP. p <0.05. p <0.05.

kimball
Télécharger la présentation

Supplemental Figure 4

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Supplemental Figure 4 a Succinate Succinate+ADPSuccinate+OligoSuccinate+FCCP b Succinate Succinate+ADPSuccinate+OligoSuccinate+FCCP p<0.05 p<0.05 Figure S4: Bioenergetics of mitochondria from liver (A) and skeletal muscle (B) using succinate as a substrate. Bioenergetic determinations were performed with the Seahorse XF24. Mitochondria (10 μg) were attached to the surface of TC plates and then treated sequentially with ADP (final concentration; 0.1 mM), oligomycin (Oligo; 2.5 μg/mL), FCCP (8 μM), and antimycin A (4 μM) to test state 3, state 4, maximal respiration, and respiration independent of the electron transport chain, respectively. 1 μM rotenone was included in the reaction buffer to prevent reverse electron flow from complex II to complex I. OCR values were corrected for respiration not associated with the electron transport chain (antimycin A)Student t-test, n=4, mean±SEM.

More Related