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Data Collection for David Gates Cezanne & Taylor

Data Collection for David Gates Cezanne & Taylor. David’s PNAS Catalytic Rxn (A+B->A+C). d4. T. d2. B. A. d1. d4. T. d1. T. d5m. d2. DG1s. Fuel-s. T. T. 2 nt clamp, comp to d5. C. A. d3. d1. d5m. T. d4. T. DG2-AC. d3. d3. Rep. Courtesy of Matt Olson, 2010.

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Data Collection for David Gates Cezanne & Taylor

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  1. Data Collection for David GatesCezanne & Taylor

  2. David’s PNAS Catalytic Rxn (A+B->A+C) d4 T d2 B A d1 d4 T d1 T d5m d2 DG1s Fuel-s T T 2 nt clamp, comp to d5 C A d3 d1 d5m T d4 T DG2-AC d3 d3 Rep. Courtesy of Matt Olson, 2010

  3. Courtesy of Matt Olson, 2010 A+B->A+C

  4. 1 2 3 4 5 6 X 7 8 9

  5. Gates for A1 + B1 -> B1 + C2 A1 B1 Backward B Out B Gate B (GB) Helper BC Translator Gate BC (TGBC) C2 B1 Reporter Gate C (RGC)

  6. 1 2 3 4 5 6 7 8 9

  7. Gates for A1 + B1 -> B1 + B2 A1 B1 Backward B Out B Gate B (GB) Helper B Translator Gate B (TGB) B2 B1 Reporter Gate B (RGB)

  8. 1 2 3 4 5 6 7 8 9 10 11

  9. A_1: CACAATACTATCATCAATCCCTCACTCTTCAAACCTCTAACCCTB_1: CATTCAAATCTCACCTTTCCCA CTAACAACTCTCTAC TAACCCAB_2: CCATTCATTCTCTCC TTTCCCA CTAACAACTCTCTAC TAACCCAC_2: CCTTCTTACACCTTC ATACCCTCTCATTCCTCACAAC ATTCCCABackward B: CTAACAACTCTCTACTAACCCAAATCCCTOut B: CACTCTTCAAACCTC TAACCCT CATTCAAATCTCACC TTT Gate_bot B: TGAGGGTTA GAGGTTTGAAGAGTG AGGGATTTGGGTTAGTAGAGAGTTGTTAG TGGGAAAHelper B: CCCACTCCATTCATTCTCTCCTTTCCCA Trans_bot B: AGTGGGAAAGGAGAGAATGAATGGAGTGGGAAAGGTGAGATTTGAATGAGGGTTAHelper BC: CCCACTCCTTCTTACACCTTCATACCCT Trans_bot BC: AG AGGGTATGAAGGTGTAAGAAGGAGTGGGAAAGGTGAGATTTGAATGAGGGTTARep_top B: CTAACAACTCTCTACTAACCCACCCAA/3IAbRQSp/Rep_bot B: /56-ROXN/ TTGGGTGGGTTAGTAGAGAGTTGTTAGTGGGAAARep_top C irr: CTCATTCCTCACAACATTCCCA/3IAbRQSp/Rep_bot C irr: /5TYE563/ TGGGAATGTTGTGAGGAATGAGAGGGTAT

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