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This document outlines the protocols for the storage and access of muscle biopsy and DNA samples, including procedures for sample requests and approvals for tanks of ultra-cold freezers. Samples are preserved at -80°C in liquid nitrogen and -20°C in specialized freezers to ensure their integrity. It details the reception, treatments, and storage methods for frozen tissues and cell cultures, including myoblasts and myotubes. Additionally, it covers data management, DNA/RNA extraction, mutation detection, and molecular diagnosis, ensuring compliance with laboratory standards.
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STORAGE AND ACCESS TO THE BANK MUSCLE BIOPSY DNA SAMPLES SAMPLE REQUEST TANK OF ULTRA COMPUTER FREEZER REQUEST LIQUID FREEZER -20° C APPROVAL NITROGEN -80° C FROZEN TISSUES CELL CULTURES DATA BANK DNA/RNA MORPHOLOGY MYOBLAST MOLECULAR BIOCHEMISTRY FIBROBLAST BIOLOGY PROTEIN ANALYSIS CYBRYD PRENATAL DIAGNOSIS SAMPLES PROVIDED TO LABORATORIES
? INCOMING MUSCLE BIOPSY SAMPLES ULTRA FREEZER –80°C TANK OF LIQUID N2 COMPUTER FROZEN TISSUES CELL CULTURES DATABASE MUSCLE MORPHOLOGY, IMMUNOHISTOCHEMISTRY Myoblasts, myotubes BIOCHEMISTRY, PROTEIN ANALYSIS Myotubes PRENATAL DIAGNOSIS
INCOMING DNA SAMPLES FREEZER –20°C COMPUTER RECEPTION, TREATMENT, STORAGE DATA BANK DNA/RNA EXTRACTION * MUTATION DETECTION, MOLECULAR DIAGNOSIS
OUTCOMING SAMPLES WRITTEN REQUEST ON TELETHON FORMS DELIVERY OF SAMPLES
