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Comparison of Agrobacterium-Mediated Transformation for Root-Specific Promoter Analysis in Soybean

This study compares various Agrobacterium-mediated transformation methods for analyzing root-specific promoters in soybean. By investigating the histochemical properties of different transgenic soybean lines containing ProCaMV35S:GUS fusions, we provide insight into the efficacy of low and high pressure conditions on gene expression. The findings contribute to our understanding of promoter activity in root biology, essential for enhancing soybean traits beneficial for agricultural applications.

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Comparison of Agrobacterium-Mediated Transformation for Root-Specific Promoter Analysis in Soybean

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  1. Full title of paper: A comparison study of Agrobacterium-mediated transformation methods for root-specific promoter analysis in soybean Journal name: Plant Cell Reports (PCR) Authors name: Caifeng Li, Haiyan Zhang, Xiurong Wang* and Hong Liao Address: State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, South China Agricultural University, Guangzhou 510642, China E-mail address of correspondence author: xrwang@scau.edu.cn

  2. p35S-17 p35S-22 p35S-31 HP LP Online Resource 4. Histochemical analysis of different transgenic soybean lines containing ProCaMV35S:GUS fusions in pTF102 under both LP and HP conditions.Soybean seeds were treated with LP (5 µmol/L) or HP (250 µmol/L) from seed germination. P35S-17, P35S-22 and P35S-31 represents pTF102-CaMV35S:GUS transgenic plants.

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