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This overview delves into the intricate world of biotechnology, emphasizing the techniques used in DNA manipulation, genetic engineering, and genomics. Key practices include employing restriction enzymes for DNA cutting, gel electrophoresis for mapping, and Polymerase Chain Reaction (PCR) for DNA replication. Discover the concepts behind cloning, recombinant DNA technology, and the creation of transgenic organisms. The importance of gene therapy, genetic counseling, and screening in addressing genetic disorders is also highlighted, showcasing the profound impact of biotechnology on medicine and agriculture.
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Biotechnology Overview Create a Frayer Diagram graphic organizer
Manipulating DNA Genomics Biotechnology Genetic Engineering Genetic Screening Diagram layout
Cut DNA with restriction enzymes • At specific locations • Gel electrophoresis creates maps of the DNA • Polymerase Chain Reaction (PCR)- copies DNA • DNA Fingerprint – molecular identification for an individual • Forensics, Paternity and kinship, study biodiversity changes. Manipulating DNA
Adding or changing DNA • Cloning • Genetically identical copy • Plants clone through regeneration; animals clone via nuclear transfer • Adding Genes • Recombinant DNA – most often uses bacterial plasmids • Transgenic organisms – contains genes from multiple organisms (i.e. GMOs) • Gene Knockout – Turning off specific genes Genetic Engineering
Study of Genomes • Gene sequencing – maps the order of the nucleotide sequence • Human Genome Project • Completed in 2003 (nucleotide order) • Today – locating the specific locations of genes on chromosomes • Bioinformatics – analyze biodata • DNA microarrays – analyzing many genes at once • Proteomics – study and compare protein structures based on gene information Genomics
Determine the risk of having or passing on a genetic disorder • Gene Therapy – Replacing defective or missing genes • Use stem cells (bone marrow) • “suicide” genes in cancer cells • Genetic Counseling – Use information from gene sequencing to counsel individuals about genetic risk factors. Genetic Screening