1 / 15

IL-1 Induces EGFP Expression and RelA Translocation in HeLa Cells: Insights into Signal Transduction

This study explores the induction of EGFP expression in HeLa cells transiently transfected with a pIL8 reporter following stimulation with 1 nM IL-1. We observe the nuclear/cytoplasmic distribution of RelA (p65), highlighting cell-to-cell variations in signal transduction amplitude and kinetics. These variations create significant noise in the signaling system. We aim to identify fundamental controlling parameters at the single-cell level to enhance the understanding of regulatory mechanisms, paving the way for more accurate mathematical modeling of signaling networks and improved experimental designs.

lyle
Télécharger la présentation

IL-1 Induces EGFP Expression and RelA Translocation in HeLa Cells: Insights into Signal Transduction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Transmembrane Signal Transduction Inflammatory mediators

  2. Induction of EGFP expression by 1 nM IL-1 in HeLa cells transiently transfected with a pIL8 reporter Minutes after IL1 IL1 vehicle

  3. EGFP Expression from pIL8-d2-EGFP in ten individual cells following IL-1 (1 nM) stimulation

  4. RelA Nuclear/CytoplasmicDistribution Response to IL-1

  5. RelA Nuclear/CytoplasmicDistribution Heterogeneity

  6. Kinetic Analysis of IL-1 Induced p65/relA Nuclear Translocation

  7. Comments Cell to cell variations in both amplitude and kinetics of signal transduction events cause a significant noise in the system. Obtaining detailed information of regulatory mechanisms at the single cell level requires identification of fundamental controlling parameters. -------------- To use the single cell data as the basis for mathematical modelling of signalling networks. To use the theoretical predictions for improved design of the biological experiment.

More Related