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Supplemental Figure 1 Chromosomal location of the pyrosequencing primers designed for FOXA1

Supplemental Figure 1 Chromosomal location of the pyrosequencing primers designed for FOXA1. FOXA1a. FOXA1b. FOXA1c. Transcription start site. FOXA1a: (Chr14: 37132056 to 37131970) CAA CG ATTTCCCCCCAAAGGG CG CTTTGC CG AGGCTGGTTGGGGTGGAGACTTGGGATAC CG CCTGG CG TG GATGGAGGAGG CG AT. 1.

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Supplemental Figure 1 Chromosomal location of the pyrosequencing primers designed for FOXA1

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  1. Supplemental Figure 1 Chromosomal location of the pyrosequencing primers designed for FOXA1 FOXA1a FOXA1b FOXA1c Transcription start site FOXA1a: (Chr14: 37132056 to 37131970) CAACGATTTCCCCCCAAAGGGCGCTTTGCCGAGGCTGGTTGGGGTGGAGACTTGGGATACCGCCTGGCGTG GATGGAGGAGGCGAT 1 2 3 5 4 6 FOXA1b: (Chr14: 37131940 to 37131842) TGGCGTGTAAGGCGATTACACATCTTCCTGGGGTCATATTTTCTGTGAGCGTTTATCTTTCATGCCTGCCGCGC TAGGATTTTCCCCCTGCCGTGCAA 7 8 9 10 11 12 FOXA1c: (Chr14: 37131831 to 37131732) TCGGTCTGGGGAGGGCGCTTAGCCATTTGCCCCGGGTGGGGGCGTCCCGTGTTCTGCAAAGTGCTCT GCACTTGCCCCCGGGTTAGG 13 14 15 16 17 18

  2. Supplemental Figure 2 Chromosomal location of the pyrosequencing primers designed for FOXB2 FOXB2a FOXB2b Transcription start site FOXB2a: (Chr9: 78824247 to 78824291) GAGGGTTTGGAGGGCGAGAGGGCAAAGTGGCGGGACTGGAGGGGCCG 1 2 3 FOXB2b: (Chr9: 78824591 to 78824689) AACGACTGCTTCATCAAGATTCCGCGGAGGCCCGACCAGCCTGGCAAGGGTAGCTTCTGGGCGCTGCA CCCCGACTGCGGGGACATGTTCGAGAACGG 4 6 7 8 5 9 10 11 12

  3. Supplemental Figure 3 Chromosomal location of the pyrosequencing primers designed for BCAR3 BCAR3a BCAR3b Transcription start site BCAR3a : (Chr1: 93799031 to 93798997) GTGTTGCTCCCTCTGCGTCGCGGTGGGGAGAGGCG 1 2 3 4 BCAR3b : (Chr1: 93799831 to 93799800) CGCGAATCGGCTCGCCGGGGTGGGGCCGCGG 5 6 7 8 9 10 11

  4. Supplemental Figure 4 Pyrosequencing data showing hypymethylation at specific CpG islands of the FOXB2 and BCAR3 loci FOXB2b FOXB2a 20 Parous 30 Nulliparous 15 20 10 10 5 0 0 4 5 6 7 8 9 10 11 12 CpG site 1 2 3 BCAR3a 10 BCAR3b 3 Parous Nulliparous 8 2 6 % methylation % methylation 4 1 2 0 0 CpG site 1 2 3 4 5 6 7 8 9 10 11

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