1 / 15

Innovations in Genome Recoding: Engineering Life and Immunity

This presentation by Peter Carr, delivered on August 18, 2011, in Lima, Peru, explores the groundbreaking techniques in genome recoding. It discusses the potential of DNA engineering to create novel life forms, develop targeted vaccines, and enhance gene therapy. Key highlights include the Multiplex Automated Genome Engineering (MAGE) method, which allows for precise modifications across genomes, creating "genetic firewalls" against viruses. The advancements in synthetic biology pave the way for new biochemistry, metabolic pathways, and personalized medicine, raising the question: How big can we go?

mayes
Télécharger la présentation

Innovations in Genome Recoding: Engineering Life and Immunity

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Recoding the Genome Peter Carr 18August 2011 FAB7 Lima, Peru

  2. 103 104 105 106 DNA On-Demand rewrite genomes minimal life genetic circuits metabolic pathways DNA base pairs vaccines gene therapy individual genes assembly scaffolds

  3. 10000000 1000000 100000 10000 1000 100 10 1 1950 1960 1970 1980 1990 2000 2010 2020 How big can we go? biology nucleotide bonds biochemistry organic chemistry year Carr & Church (2009) Nature Biotech.

  4. The basic genetic code 4 letters 4 letters 4 letters 64 words (codons) A C G T A C G T A C G T = AAA AAC AAG AAT ACA ACC ACG ACT ... X X

  5. Second Position

  6. By engineering new genetic codes we can: Second Position new room for plug & play rearrange to block function • make universal systems for “plug and play” with new amino acids • block exchange of genes, a “genetic firewall” • produce organisms immune to viruses

  7. Multiplex Automated Genome Engineering (MAGE) “Find and replace” with the DNA of the genome

  8. Key features of MAGE: • Multiplexed: can address many sites at once • Targeted: modifies specific sequences; changes made can be specific or random • Iterated: weak efficiency can be compensated by automating many cycles of modification • Tunable: can control the range of diversity generated in a population • Clean: no DNA scars, no resistance gene markers, etc.

  9. All 314 sites in the genome modified (across 32 strains)

  10. Genome Engineeringbase pairs to megabases

  11. Joseph Jacobson Bram Sterling* Chris Emig Sam Hwang George Church (HMS) FarrenIsaacs Harris Wang Marc LaJoie ShuguangZhang (MIT) Thanks to: Funding: NSF

  12. A T A C A G T genetic counseling molecules health statistics me you forensics it basic research experiments Atoms to bits...to atoms to bits transmitter receiver Box 1 Box 2 clinical + research data

  13. Box 2 MY TREATMENT Personal Box 1 MY GENES How will drugs X, Y, Z affect ME? How will MY BODY metabolize drugs X, Y, Z?

  14. Box 2 RAPID RESPONSE Community Box 1 NEW PATHOGEN Analyze the PARTS Test existing DRUGS at many sites Preliminary VACCINE

  15. Box 2 MY DESIGN FOR... Creativity MY IDEA new vaccines new biochemistry novel organisms DNA nanostructures

More Related