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This study utilizes the Trypan Blue cell viability assay to assess the proportion of live and dead cells in 6-well plates, comparing two experimental setups. We present data on live cell counts (x10,000) and dead cell percentages post-DMSO treatment across varying concentrations. Statistically significant differences (p < 0.05) were observed when compared to control conditions, providing insights into the cytotoxic effects of DMSO. These findings contribute to our understanding of the cell viability under specific chemical influences.
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Supplemental Fig. 1 Live and dead cells estimated by trypan blue cell viability assay in 6-well plates. A Experiment 1 A’ Experiment 2 d8 d6 d7 d4 d8 d1 d4 d2 d3 Live cells number (x10000) Live cells number (x10000) d1 * * * * * * * * 0 0.5 1.25 2.50 0.5 1.25 2.50 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 % DMSO treatment 0 0.5 1.25 2.50 0.5 1.25 2.50 0.5 1.25 2.5 0 0.5 1.25 2.50 0.5 1.25 2.5 % DMSO treatment B B’ d2 d8 * d1 d8 d3 d4 d4 * d1 * * d6 d7 * Dead cells (% of total cells) Dead cells (% of total cells) * *p<0.05 in comparison with control 0 0.5 1.25 2.50 0.5 1.25 2.50 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 % DMSO treatment 0 0.5 1.25 2.50 0.5 1.25 2.50 0.5 1.25 2.5 0 0.5 1.25 2.5 0 0.5 1.25 2.5 % DMSO treatment *p<0.05 in comparison with control
