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Cell Biology 13 - cytoskeleton 3 rd

Cell Biology 13 - cytoskeleton 3 rd. Sung Youn Lee, PhD. Student Veterinary collage, Room 320 02 450 3719, 016 293 6059 leevet@paran.com. Spectrin Membrane Skeleton.

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Cell Biology 13 - cytoskeleton 3 rd

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  1. Cell Biology 13- cytoskeleton 3rd Sung Youn Lee, PhD. Student Veterinary collage, Room 320 02 450 3719, 016 293 6059 leevet@paran.com

  2. Spectrin Membrane Skeleton The walls of mature red blood cells are made tough and flexible because of skeletal proteins like spectrin and actin, which form a network with the aid of protein 4.1.

  3. Biconcave shape

  4. Intermediate filament • Diameter : 10nm • Thickness : microfilament < Intermediate filament < microtubule • To provide resistance to mechanical stress placed upon the cell • Composed of heterogeous group of proteins • Microfilament ; actin • Microtubule ; tubulin • Intermediate filament ; several proteins

  5. IF monomer Parallel dimer Two dimer forms antiparallel tetramer Two dimer forms antiparallel tetramer  staggered 8 tetramer  protofilament Intermediate filament -rope like structure

  6. GFAP

  7. GFAP- Rat HippocampusTrimethyl Tin Intoxication

  8. Microtubules • are straight, hollow cylinders • have a diameter of about 25 nm • are variable in length but can grow 1000 times as long as they are thick • are built by the assembly of dimers of alpha tubulin and beta tubulin. • are found in both animal and plant cells

  9. Microtubules • [Function] • Ciliary and flagella motility • mitotic and meiotic chromo- • somal movements • intracellular vesicle transport • secretion • other processes

  10. Tubulin • Drugs have effect on tubulin • Colchicine • Vinblastin sulfate • Nocodazole • Paclitaxel(Taxol) Disrupt the nomal dynamic behavior of microtubule Cell vidision ▽ Anti-cancer drug

  11. GTP assembling GDP disassembling

  12. The neuronal cytoskeleton • Polymorphism • Directional flow of information

  13. The shape of neurons is determined by their cytoskeleton • EM-neuronal cytoplasm is filled with cytoskeletal structure. • Axial plane : 1st microtubule, 2nd neurofilament, 3rd actin microfilament – at the tip • Cytoskeletal structure plays important role in constructing the unique neuronal morphology.

  14. Neuronal metabolism requires stable and dynamically unstable filamentous structure • Neurons elongate and retract their process and can change their patterns of connectivity throughout life. • This is made possible by the existence of actin and tubulin in both a monomeric and polymeric state within neurons.

  15. Fast axonal transport is a major form of motility for secretory and membranous components • Average rate ; 250~400mm/day • Membrane associated • Plasmalema (proteins, cholesterol, phospholipids, gangliosides), synaptic vescle (neurotranmitters, neuropeptides, and enzymes), and mitochodria (50mm/day)

  16. Movement of the cytosol occurs by slow axonal transport • Average rate ; 1mm/day • Fibrillar elements in the cytoskeleton

  17. Thank you for your attention ~

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