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Organizing the cell: the Cytoskeleton

Organizing the cell: the Cytoskeleton. Intermediate Filaments. Microfilaments. Microtubules. Microtubules. Text and image sources are included using the notes function of this file. EM views. FtsZ: a prokaryotic ur-tubulin?. The Tubulin Superfamily. Microtubule model. exchangeable. GDP.

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Organizing the cell: the Cytoskeleton

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  1. Organizing the cell: the Cytoskeleton Intermediate Filaments Microfilaments Microtubules

  2. Microtubules Text and image sources are included using the notes function of this file

  3. EM views

  4. FtsZ: a prokaryotic ur-tubulin?

  5. The Tubulin Superfamily

  6. Microtubule model exchangeable GDP GTP “Minus” end “Plus” end GTP GTP GTP GTP GDP GTP GTP GTP GDP GDP GDP GDP GDP GDP GTP GTP GTP GTP GTP GTP GTP GTP GDP GTP GTP Beta-tubulin “cap”

  7. Plus Minus Tubulin dimer atomic structure In Out

  8. Microtubule fly-by Narrated Video

  9. Microtubule initiation

  10. MTOCs

  11. The Centrosomerevealed 9-triplet microtubule centrioles pericentriolar material singlet microtubules

  12. Washout of nocodazole 10 um Successive images at 2 minute intervals after changing to drug-free media (at timepoint A)

  13. Dynamic Instability in vitro Video

  14. Dynamic instability in vivo Video

  15. Fluorescence Speckle Microscopy Video

  16. Disassembly Stathmin microtubule-binding domain Video In vitro assembly on axonemal microtubule seeds +/- Stathmin Activity

  17. Katanin Video Microtubule wall (Mitotic Extract) (Interphase Extract)

  18. Centrosomal release

  19. Treadmilling Speckles Photobleaching

  20. MAPs Note the cross-bridges (arrows) between microtubules

  21. Plus end tracking proteins (+TIPS) Video Video GFP::EB1 Cy3 Tubulin um

  22. Interrelationship between microtubules and microfilaments Microtubule effects Microfilament effects Video Video

  23. Synthesis and Folding Signal transduction Integration with actin networks cof B cof E cof C CCT cof D cof A Pathways stathmin PAK Rac Cdc42 a b Rho IQGAP abGTP • Tub; CLIP-170, EB-1 mDia GEF? Dynamic Polymer Rho stabilizing MAPs; + end capture stathmin catastrophic kinesins abGDP katanin severin (EF1a) StablePolymer Assembly/Disassembly

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