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Calculating Magnification and Size of Organelles from Micrographs

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This document outlines the process to measure and calculate the size of cellular organelles using micrographs. With an example of a micrograph magnified at X100,000, the measured image size is converted to micrometers (µm). By converting the measured size of 0.85 mm to µm, we find it equals 85,000 µm. Furthermore, the actual diameter of the organelle is stated as 0.5 mm, allowing us to compute the magnification of the diagram, which is determined to be X20,000, demonstrating the relationship between actual size and observed magnification.

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Calculating Magnification and Size of Organelles from Micrographs

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  1. Measuring Micrographs Work out the sizeof an organelle in m Measured image size Magnification Magnification of micrograph is X100000 Measured size = 85mm Convert to m = 85000  85000 m 100000 = 0.85m or 850nm

  2. 0 1 2 3 4 5 6 7 8 9 10

  3. 0 1 2 3 4 5 6 7 8 9 10

  4. 0 1 2 3 4 5 6 7 8 9 10 The actual diameter of organelle Y is 0.5 mm. Calculate the magnification of the diagram. Show your working.

  5. 0 1 2 3 4 5 6 7 8 9 10 X 20,000

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