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No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs

No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs. HRI-/- #3. WT #7. HRI-/- #5. WT #11. Tg60’. Tg15’. Tg15’. Tg30’. Tg60’. Tg30’. Tg60’. Tg30’. Tg15’. Tg30’. Tg60’. Tg15’. As30’. As15’. As30’. As60’. As60’. As15’. As30’. As60’. As15’. As30’. As60’. As15’. UT. UT.

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No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs

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  1. No (apparent) defect in eIF2a(P) in As-Rx’d HRI-/- MEFs HRI-/- #3 WT #7 HRI-/- #5 WT #11 Tg60’ Tg15’ Tg15’ Tg30’ Tg60’ Tg30’ Tg60’ Tg30’ Tg15’ Tg30’ Tg60’ Tg15’ As30’ As15’ As30’ As60’ As60’ As15’ As30’ As60’ As15’ As30’ As60’ As15’ UT UT UT UT eIF2a(P) eIF2a(T) As: Sodium arsenite, 100M, Tg: Thapsigargin, 100nM Isabel Novoa & Yuhong Zhang

  2. Genotyping of HRI locus HRI-/- #3 HRI-/- #5 HRI-/- #3 HRI-/- #5 WT WT X174 HaeIII 1.3Kb MUT (1.1Kb) 1.1Kb 0.9Kb WT (0.6 Kb) 0.6Kb WT allele (P2/P6 PCR) MUT allele P2/P4 PCR

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