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Media untuk Kultur Jaringan Tanaman

Media untuk Kultur Jaringan Tanaman. Fungsi media. Menyediakan air Menyediakan kebutuhan hara mineral Menyediakan vitamin Menyediakan zat pengatur tumbuh Akses ke atmosfer untuk pertukaran gas Membuang sisa metabolisme tanaman. Unsur – unsur Mineral. Unsur Makro. Nitrogen (N)

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Media untuk Kultur Jaringan Tanaman

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  1. Media untuk Kultur Jaringan Tanaman

  2. Fungsi media • Menyediakan air • Menyediakan kebutuhan hara mineral • Menyediakan vitamin • Menyediakan zat pengatur tumbuh • Akses ke atmosfer untuk pertukaran gas • Membuang sisa metabolisme tanaman

  3. Unsur – unsur Mineral

  4. Unsur Makro • Nitrogen (N) • ion nitrate (NO3- oxidized) • ion ammonium (NH4+ tereduksi) • 25-60 mM • organik

  5. Unsur makro • Kalium (K)20 -30 mM • Fosfor (P) 1-3 mM • Kalsium (Ca)1-3 mM • Magnesium (Mg) 1-3 mM • Sulfur (S) 1-3 mM

  6. Hara mikro • Besi (Fe) 1 m M • Mangan (Mn) 5-30 m M • Seng (Zn) • Boron (B) • Tembaga (Cu) 0.1 m M • Molybdenum (Mo) 1 m M • Cobalt (Co) 0.1 m M • Iodine (I) Nickel (Ni), aluminum (Al), and silicon (Si)

  7. Senyawa Organik • Gula • sukrosa • lainnya • 20 to 40 g/l • Vitamin • thiamin (vitamin B1) • nicotinic acid (niacin) dan pyridoxine (B6) • myo-inositol

  8. Senyawa lain • Arang aktif • Zat pengatur tumbuh!

  9. Sistem pendukung • Agar (dari rumput laut) • Agarose • Gelrite (Phytagel) (dari backteri) • Campuran (Phytagar) • Mekanik (bridges, rafts) • Pasir

  10. Formulasi Media • Banyak tersedia • Berbeda pada konsentrasi garam • Berbeda pada ada dan tidaknya garam

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