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ANNOUNCEMENTS

ANNOUNCEMENTS. Research Lab Technician position: An entry level Research Assistant position or above is available immediately, assisting in study of the role of mitochondrial dysfunction in disease using cellular and animal models. Candidates with expertise in mammalian cell culture, molecular

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ANNOUNCEMENTS

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  1. ANNOUNCEMENTS Research Lab Technician position: An entry level Research Assistant position or above is available immediately, assisting in study of the role of mitochondrial dysfunction in disease using cellular and animal models. Candidates with expertise in mammalian cell culture, molecular biological and biochemical techniques, are strongly encouraged to apply. Additionally, candidate must be willing to work with animals (mice & rats). Responsibilities include experiment planning & design, data analysis & interpretation, maintaining records with the help of supervisor. Candidate must be well organized, be attentive to details, have good communication skills, and be able to work effectively in a team environment. Requirements: A Bachelors degree is required, preferably within molecular biology or related discipline. Knowledge and experience with animals (especially mice) is critical. etc. For more information or to submit a resume: www.baystatehealth.com/jobs Keyword search: Research Email: mike.neuendorf@bhs.org Fax: 413-794-3325 AA/EOE

  2. ANNOUNCEMENTS Final Exam: May 18th 1:30 PM in Morrill 203 Review Sessions: Friday 5/8 12:20 Wednesday 5/13 Timing ?? Sunday 5/17 Timing ?? Sample Exam Questions

  3. LAB SCHEDULE • Week of May 4: Immunohistochemistry. Sectioning & staining of embedded material. Staining and imaging on your own during the week/weekend. • CHOOSE ANTIBODIES (REVIEW MATERIALS) • READ STAINING PROTOCOL • READ LAB PROJECT INFORMATION • GUIDELINES FOR LAB PROJECT REPORT • In class, May 11: Share results, discuss interpretation. • May 18: Lab Project report due at Final Exam.

  4. LAB PROJECT REPORT • 3-4 page paper written in your own words • The report should include images of: • toluidine-stained frozen section • immunohistochemistry results • toluidine-stained paraffin-embedded sections • Images should be labeled and have a figure legend. • The report should answer the following questions: • What organ did you stain and why did you choose it? • What are its distinguishing histological features? • What is the function of the organ? How do the histological features contribute to its function? • What markers did you use in the immuno. experiment? • What structures did you predict would stain? What actually stained? • Discuss your results, problems or artifactual staining.

  5. LAB PROJECT REPORT • Rubric for Scoring (30 Points) • - clarity and appropriate use of histological terms (5) • - accurate, clear, and neat labeling of images, appropriate figure legends (15 points) • - discussion of choice of markers, anticipated staining, interpretation of actual staining pattern, and discussion of problems or artifactual staining (10)

  6. *BIOLOGY SENIORS* Join us for lunch! 12 Noon Wednesday, May 13th Lawn, Durfee Conservatory

  7. Thyroid Thyroid Follicles Epithelium: simple cuboidal or low columnar Follicular Cells - produce thyroid hormone T3 and T4 - spherical nuclei - basophilic - microvilli Parafollicular ( C ) Cells - produce calcitonin - follicule periphery - within basal lamina - pale staining Fenestrated capillaries

  8. Follicular Cells Arranged around lumen filled with acidophilic colloid (PAS positive)

  9. Parafollicular Cells • Calcitonin: • Calcitonin secreted in response to increase in blood calcium • Suppresses osteoclast function, increase osteiod calcification • Decreases blood calcium levels • Physiological antagonist to parathyroid hormone

  10. THYROID: Follicular and Parafollicular cells

  11. Thyroid Hormone Synthesis 1) Follicular cells produce thyroglobulin and secrete it into lumen Follicular cells actively take up iodide and it is oxidized to iodine and released into the colloid. Tyrosine residues on thyroglobulin are iodinated to form MIT and DIT. Mediated by thyroid peroxidase on apical surface. MIT= monoiodotyrosine DIT= diiodotyrosine

  12. Thyroid Hormone Synthesis MIT + DIT form T3 DIT + DIT form T4 Still part of thyroglobulin in colloid

  13. Thyroid Hormone Synthesis 5)Upon stimulation with TSH (Thyroid Stimulating Hormone), thyroglobulin is reabsorbed by receptor mediated endocytosis and combine with lysosomes, releasing T3 and T4.

  14. Thyroid Hormone Synthesis Metabolic Activity: T3 >>> T4 Amount released from Thyroid: T4 >>> T3 20:1 Transport in blood: Bound to transport (carrier) protein --> Thyoxine-binding protein T4 is converted to T3 in kidneys and liver

  15. Thyroid Hormone Targets and Function - Increases basal metabolic rate (BMR) (heat production) - Accelerates protein synthesis in children body growth and development brain development - Enhances sympathetic tone

  16. Thyroid Hormone Targets and Function Thyroid Hormone Receptor: intracellular, bound to DNA as repressor without T3; with T3 present, acts as an activator of gene transcription

  17. Thyroid Hormone Release: Negative Feedback Thyroid Releasing Hormone (TRH) Thyroid Stimulating Hormone (TSH) Thyroid Hormones (T3 and T4)

  18. Diseases of the Thyroid Iodine Deficiency: low T3 + T4, elevated TSH, goiter Hypothyroidism

  19. Diseases of the Thyroid Hypothyroidism

  20. Diseases of the Thyroid Hyperthyroidism

  21. Diseases of the Thyroid Graves Disease

  22. Graves Disease: T3 and T4 TSH

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