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Comprehensive Primer Cut Sequences for pSB1 and I13504 in Riboswitch Applications

This document outlines various cut sequences and primer designs used in the ligation of constructs involving the pSB1(A2/A3/AK3) series and I13504. It details specific cut locations for multiple primers, including riboswitch sequences, Lox sites, and several unknown primers to facilitate genetic engineering applications. This structured overview offers valuable insights into the manipulation of these plasmids, supporting synthetic biology initiatives and experimental design.

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Comprehensive Primer Cut Sequences for pSB1 and I13504 in Riboswitch Applications

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  1. Ligation Chart C0261 (24A) Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 (12D) Cut: X & P Primer: I13504 F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) C0261 + I13504 Cut: X & P Primer: I13504 Riboswitch Cut: Pre-cut Primer: Riboswitch C0261 + I13504 Cut: E & X Primer: pSB1A2 Primer (Reverse) F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) RS + C0261 + I13504 Cut: X & P Primer: I13504 Lox Sites Cut: UNKNOWN Primer: UNKNOWN C0261 + I13504 Cut: UNKNOWN Primer:UNKNOWN F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) LOX + C0261 + I13504 Cut: X & P Primer: I13504 J37019 Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 Cut: X & P Primer: I13504 B0034 (3O) Cut: S & P Primer: pSB1(A2/A3/AK3) C0062 (7A) Cut: X & P Primer: C0062 2 R0062 (9G) Cut: S & P Primer: pSB1(A2/A3/AK3) B0034 + C0062 Cut: X & P Primer: C0062 2 I13033 (2H) Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 (12D) Cut: X & P Primer: I13504 I13033 + I13504 Cut: X & P Primer: I13504 J37019 Cut: S & P Primer: pSB1(A2/A3/AK3) B0034 (3O) Cut: S & P Primer: pSB1(A2/A3/AK3) J37024 Cut: X & P Primer: UNKNOWN J37023 Cut: S & E Primer: UNKNOWN B0015 (1I) Cut: X & E Primer: UNKNOWN J04500 (16P) Cut: S & P Primer: pSB1(A2/A3/AK3) J37024 Cut: X & P Primer: UNKNOWN J37025 Cut: X &P Primer: J37024A J37019 Cut: S& P Primer: pSB1(A2/A3/AK3)

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