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Molecular Ligation Chart and Primer Guide for DNA Manipulation

Navigate the complex world of DNA ligation with this comprehensive chart and primer guide. Find detailed instructions and primers for various DNA cutting and ligation processes.

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Molecular Ligation Chart and Primer Guide for DNA Manipulation

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  1. Ligation Chart C0261 (24A) Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 (12D) Cut: X & P Primer: I13504 F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) C0261 + I13504 Cut: X & P Primer: I13504 Riboswitch Cut: Pre-cut Primer: Riboswitch C0261 + I13504 Cut: E & X Primer: pSB1A2 Primer (Reverse) F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) RS + C0261 + I13504 Cut: X & P Primer: I13504 Depends on PCR which is yet to work in our lab Lox Sites Cut: UNKNOWN Primer: UNKNOWN C0261 + I13504 Cut: UNKNOWN Primer:UNKNOWN F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) LOX + C0261 + I13504 Cut: X & P Primer: I13504 J37019 Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 Cut: X & P Primer: I13504 B0034 (3O) Cut: S & P Primer: pSB1(A2/A3/AK3) C0062 (7A) Cut: X & P Primer: C0062 2 R0062 (9G) Cut: S & P Primer: pSB1(A2/A3/AK3) B0034 + C0062 Cut: X & P Primer: C0062 2 I13033 (2H) Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 (12D) Cut: X & P Primer: I13504 I13033 + I13504 Cut: X & P Primer: I13504 J37019 Cut: S & P Primer: pSB1(A2/A3/AK3) B0034 (3O) Cut: S & P Primer: pSB1(A2/A3/AK3) J37024 Cut: X & P Primer: UNKNOWN Waiting on immuno tag Depends on PCR which is yet to work in our lab J37023 Cut: S & E Primer: UNKNOWN B0015 (1I) Cut: X & E Primer: UNKNOWN J04500 (16P) Cut: S & P Primer: pSB1(A2/A3/AK3) J37024 Cut: X & P Primer: UNKNOWN Cre Cut: X & P Primer: UNKNOWN J04500 (16P) Cut: S & P Primer: pSB1(A2/A3/AK3) J37023 Cut: S & E Primer: UNKNOWN B0015 (1I) Cut: X & E Primer: UNKNOWN

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