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Enhancing Streptavidin Binding with Icmt Substrate for Improved Bioconjugation

Discover how Icmt substrate boosts Streptavidin binding efficiency, leading to higher Icmt activity and enhanced bioconjugation.

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Enhancing Streptavidin Binding with Icmt Substrate for Improved Bioconjugation

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  1. A. O H N N H 2 3 2 4 2 5 2 1 2 2 H 2 0 1 9 N 1 4 1 7 1 5 1 2 1 0 8 6 4 2 1 3 S S 1 8 1 6 1 1 9 7 5 3 1 O COO- Streptavidin binding Icmt substrate B. 90 80 70 60 50 Icmt activity (pmol.hr-1.mg-1) 0.20  0.18 40 0.16 0.14 0.12 1/rate (pmol-1.hr.mg) 30 0.10   0.08  0.06  20  0.04     0.02 0.00 10 1/[BFC] (mM-1) 1 2 3 4 5 6 0 2 4 6 8 10 12 14 [BFC] (mM)

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