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Higher Content Yeast Imaging and Data Processing

Higher Content Yeast Imaging and Data Processing. Richard Alexander Stowers Microscopy Center May 19, 2009. High Content Imaging Candidates. Reproducible Segmentable Automatable Acquisition Analysis. High Content Imaging Candidates. Reproducible Segmentable Automatable Acquisition

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Higher Content Yeast Imaging and Data Processing

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  1. Higher Content Yeast Imaging and Data Processing Richard Alexander Stowers Microscopy Center May 19, 2009

  2. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  3. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  4. Control Plate Experimental Plate

  5. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  6. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  7. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  8. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  9. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  10. High Content Imaging Candidates • Reproducible • Segmentable • Automatable • Acquisition • Analysis

  11. Use VBA Code to… • Group Colonies • Classify Colonies • Compare between control and experimental plates

  12. Use VBA Code to… • Group Colonies • Classify Colonies • Compare between control and experimental plates

  13. A B C • Use VBA Code to… • Group Colonies • Classify Colonies • Compare between control and experimental plates 1 2 3

  14. Summary • Yeast Robot • Pin yeast plate • Flatbed Scanner • Image yeast plate • Axiovision • Automate Segmentation • Automate Data Acquisition • Excel • Automate Data Processing

  15. Acknowledgements • Rong Li • Das Arupratan • Brian Slaughter • Molecular Biology • Dan Bradford • Brian Sanderson

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