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Translation

This resource provides an overview of tRNA structure and function in the translation process. It covers essential details such as the number of RNA strands in tRNA, the locations where amino acids attach, and the types of bonding present. Additionally, it discusses the types of tRNA, the role of aminoacyl-tRNA synthetase, and the components of the translation initiation complex. Engaging questions related to these topics facilitate deeper understanding of the mechanisms involved in protein synthesis.

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Translation

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Presentation Transcript

  1. Translation Obviously Not Transcription ,Transduction , or Transformation

  2. ProkvsEuk

  3. How many strands of RNA make up a tRNA? • 1 • 2 • 3 • 4 • 5

  4. What is being represented in the red, yellow, green and blue portions of this tRNA? • Hydrogen bonding • Ionic bonding • Nucleotide modification • Disulfide bonds • snRNP’s

  5. Where does an amino acid attach to? • 5’ end • D loop • Anticodon • T loop • 3’ end

  6. What amino will be “loaded” onto the attachment site • Threonine • Tryptophan • Lysine • Phenyalanine • Leucine

  7. What amino will be “loaded” onto the attachment site • Threonine • Alanine • Lysine • Arginine • Glycine

  8. What amino will be “loaded” onto the attachment site • Phenylalanine • Leucine • Glutamic acid • Lysine • Glycine

  9. How many different types of tRNA’s are there? • 10 • 20 • 45 • 61 • 64

  10. What is the name of the enzyme shown below? • Peptidyl tRNA Transferase • Amino-acyl tRNA Synthetase • Amino Acid RNA Transferase • ATP amino acidase

  11. How does the enzyme know which amino acid to put on the 45 different tRNAs? • They have a piece of RNA that is complementary to the anticodon and amino acid. • The shape of the active site is complementary to the tRNA arm and amino acid. • An amino acid acts as an allosteric activator. • ATP allows for induced fit of tRNAs 64 different anticodons. • The nucleolus yells at it until it catalyzes the correct reaction.

  12. Which of the following is NOT a component of the translation initiation complex? • Small ribosomal subunit • tRNA with a 3’ UAC 5’ anticodon • Primary transcript that was originally transcribed by RNA polymerase II. • Large ribosomal subunit • Peptidyl site in the large ribosomal subunit.

  13. What was the energy source that brought all of these components together? • ATP • GTP • CTP • UTP • dATP • dGTP • dCTP • dTTP • FIRE

  14. How many different types of RNA are shown here? • 1 • 2 • 3 • 4 • 5

  15. Who does this piece of art resemble the most? • Mr. Steyer • Mr. Heinrich • Mr. Carnes • Mr. Fabo

  16. Amino Acid? 1 • 1 • 2 • 3 • 4 • 5 • None 4 2 3 5

  17. Amino-acyl? 1 • 1 • 2 • 3 • 4 • 5 • None 4 2 3 5

  18. mRNA? 1 • 1 • 2 • 3 • 4 • 5 • None 4 2 3 5

  19. Introns? 1 • 1 • 2 • 3 • 4 • 5 • None 4 2 3 5

  20. Animations http://www-class.unl.edu/biochem/gp2/m_biology/animation/gene/gene_a3.html http://vcell.ndsu.edu/animations/translation/index.htm

  21. 3’ GGGCCCACGGAGCACAAAAA TTTTT ATATTTTT ATAGGGCCCGAGGTGTACGTCGAGCCC TTTACCATT GCGGGGAACACATTC TTATTTCCC GCGGACCC ACATCA TCAGGGG 5’ 5’ CCCGGGTGCCTCGTGTTTTTTAAAAATATAAAAATATCCCGGG CTC CACATGCAGCTCGGGAAATGGTAACGCCCC TTGTGTAAGAATAAAGGGCGCCTGGGTGTAGTAGTCCCC 3’ Blow UP! 5’ TACGTCGAGCCC TTTACCATT GCGGGGAACACATTC TTATTTCCC GCGGACCC ACATCA TCAGGGG 3’

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