1. (L) (R) 10µm • Online Resource 2 (A) Title:Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments Authors:Liyuan Hou1, Hyunsun Park 1,2, Shigeru Okada2,3, Takeshi Ohama 1,2, * 1School of Environmental Science and Engineering, Kochi University of Technology (KUT), Tosayamada, Kochi 782-8502, Japan 2JST, CREST, 5 Sanbancho, Chiyoda, Tokyo 102-0075, Japan 3Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan Corresponding author:Takeshi Ohama E-mail: ohama.takeshi@kochi-tech.ac.jp. Protoplasma

2. (2) (1) (4) (3) 100µm Title:Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments Authors:Liyuan Hou1, Hyunsun Park 1,2, Shigeru Okada2,3, Takeshi Ohama 1,2, * 1School of Environmental Science and Engineering, Kochi University of Technology (KUT), Tosayamada, Kochi 782-8502, Japan 2JST, CREST, 5 Sanbancho, Chiyoda, Tokyo 102-0075, Japan 3Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan Corresponding author:Takeshi Ohama E-mail: ohama.takeshi@kochi-tech.ac.jp. Protoplasma • Online Resource 2 (B)