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Online Resource 4 PowerPoint Presentation
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Online Resource 4

Online Resource 4

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Online Resource 4

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  1. Online Resource 4 S10 shRNA GFP Control shRNA GFP Control SS shRNA Atg-7 SS shRNA Atg-5 SS p62 62kDa β-Actin 43kDa S11 S12 shRNA GFP Control shRNA GFP Control shRNA ATG-7 1,25D3 shRNA ATG-5 1,25D3 Online Resource 4 S10 Autophagic Flux in cells with knockdown of Atg5 or Atg7Control cells stably expressing shRNA against GFP and cells in which either ATG-5 or ATG 7 was knocked down were serum starved for 72 hours and p62 degradation was measured by western blot analysis. SS (Serum Starvation) S11/S12Sensitivity to 1,25 D3 in cells with knockdown of Atg5 or Atg7Cells were exposed to 100nm 1,25D3 and viability was determined based on trypan blue exclusion.