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+. +. +. -. +. +. A. -855. - 849. tacaaag. C.F. Hong et.al., 2009. Slug. -2000. -1000. -1663. -855. - 849. - 1657. +1. ctttgaa. tacaaag. Luc. M.Saegusa et.al., 2009. ctttgtt. -1971. - 1965. -610. -855. -849. -667. -673. -616. -316. -310. +1. tacaaag. tctttgt.

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-2000

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  1. + + + - + + A -855 -849 tacaaag C.F. Hong et.al., 2009 Slug -2000 -1000 -1663 -855 -849 -1657 +1 ctttgaa tacaaag Luc M.Saegusa et.al., 2009 ctttgtt -1971 -1965 -610 -855 -849 -667 -673 -616 -316 -310 +1 tacaaag tctttgt cctttgt attttga Luc tcaaaag ctttgca -900 -894 -982 -643 -637 Region 1 Region 3 Region 2 B C * 35 30 hOB 4 Input Relative luciferase activity Lef-1 * 2 TCF-1 TCF-4 1 2 3 0 pGL3-Slug Regions + + + hLef-1 - - + hOBs SaOS-2 MCF7 fold activation 1.4 35

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