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ENDURO Horizontal Electrophoresis Systems. Casting a Gel. Attach a buffer dam to one side of gel tray. Attach the other buffer dam to the other side of gel tray. Place gel tray on a flat surface. Casting a gel. Mix agarose buffer solution and boil Cool agarose solution to below 60ºC
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Casting a Gel Attach a buffer dam to one side of gel tray Attach the other buffer dam to the other side of gel tray Place gel tray on a flat surface
Casting a gel • Mix agarose buffer solution and boil • Cool agarose solution to below 60ºC • Pour onto gel tray • Let solution cool for ½ hour • Gently remove combs
Running the Gel • Remove the casting gates • Place the gel tray into the gel box • Overlay it with buffer • Connect the lid & attach to a power supply
Staining the Gel • Remove gel from gel tray • Place into a container with stain • Destain the gel with buffer or water
Features • Heavy duty molded chambers • Removable electrodes • Multichannel pipette compatible combs • Domed lid to prevent condensation
Heavy duty molded construction • Prevents leaking problems
Changing the electrode Turn unit over & remove screw Remove old electrode Insert new electrode & secure with nylon screw
Multichannel Combs A large number of comb are compatible with multichannel pipettes
Domed Lid Prevents condensation from falling on the gel Recaptures condensation in reservoirs