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Raw Reads

Raw Reads. Length Histogram. QA/QC Analysis (ex. FastQC ). Length & Quality Trimming. Histograms. Final QA/QC Analysis. Single- k Assemblies. Read Mapping. KOI Assignment (ex. KAAS, KOBAS). Graphical Analysis. Pairwise Unique Comparison (provided script or similar).

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Raw Reads

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  1. Raw Reads Length Histogram QA/QC Analysis (ex. FastQC) Length & Quality Trimming Histograms Final QA/QC Analysis Single-k Assemblies Read Mapping KOI Assignment (ex. KAAS, KOBAS) Graphical Analysis Pairwise Unique Comparison (provided script or similar) Assembly Analysis Assembly Statistics Length Histograms Unique Contigs Multi-k Assembly (ex. CD-HIT-EST, Oases Multi-k, VMATCH, or similar) Pairwise Unique Comparison (provided script or similar) Non-redundant Contig Set BLAST Pathway Mapping Annotation EC Assignment

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