1 / 13

Team 6: Gene Expression II Teaching Unit - Information Flow in Eukaryotes:

Team 6: Gene Expression II Teaching Unit - Information Flow in Eukaryotes: Differential Gene Expression. Dave Hawthorne. Rich Walker. Reid Compton. Mary Lipscomb. Edgar Moctezuma. Jill Sible. Lil Tong. Information Flow in Eukaryotes: Differential Gene Expression.

raiden
Télécharger la présentation

Team 6: Gene Expression II Teaching Unit - Information Flow in Eukaryotes:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Team 6: Gene Expression II Teaching Unit - Information Flow in Eukaryotes: Differential Gene Expression Dave Hawthorne Rich Walker Reid Compton Mary Lipscomb Edgar Moctezuma Jill Sible Lil Tong

  2. Information Flow in Eukaryotes: Differential Gene Expression

  3. Information Flow in Eukaryotes: Differential Gene Expression

  4. Information Flow in Eukaryotes: Differential Gene Expression Context: • Freshman Biology course No prerequisites Some majors, some non-majors • Material covered before this unit: Concept of a gene Prokaryotic gene expression Appreciate and are effective in group work • Reading assignment before class on eukaryotic gene expression

  5. Tidbit 1: To make sure everyone understands the steps of genetic information flow in eukaryotes, order the following events. Mature protein Nuclear export of RNA Polypeptide elongation Recognition of start codon Positioning and activation of RNA polymerase Termination of transcription Ribosome encounters stop codon RNA processing Protein folding RNA elongation

  6. Red Green Tidbit 2a: Anthocyanin and grape color • Anthocyanins are flavonoid pigments in plant cells that make them look red, purple or blue. • The final step in the synthesis of anthocyanin is catalyzed by the enzyme UFGT. • Green grapes lack anthocyanins due to a mutation in the MYB transcription factor.

  7. Clicker activity Based on the strip sequence, which step in the UFGT expression pathway would be affected by mutation in the MYB transcription factor? Termination of transcription Recognition of the start codon Positioning and activation of RNA polymerase Protein folding None of the above Red Green

  8. Tidbit 2b: Musclehead Analysis of the human genome indicates a single a-tropomyosin gene. However, tropomyosin protein isolated from muscle and brain are structurally different.

  9. Clicker activity Regulation at which point in the tropomyosin gene expression pathway could account for two structurally different protein products encoded by the same gene? Termination of transcription RNA processing Nuclear export of RNA Protein folding

  10. Mini lecture on points of regulation and experiments to study them. Take home assignment: Design an experimental approach to determine which point of control along the gene expression pathway accounts for the different forms of tropomyosin in muscle and brain.

  11. Next class:The a-tropomyosin gene is alternatively spliced.

  12. Diversity considerations: By allowing a range of correct answers for tidbit 1, we accommodated both linear and non-linear approaches to solving the problem. Tidbit 1 also engaged kinesthetic and visual learners. We used grapes rather than wine (alcohol) as an example. We used an example from the plant/agricultural field because not all students are pre-med. We were sensitive to potential color-blindness by pointing out different grape colors.

  13. Thank You! From the GrapeStormers

More Related