1. Hematopoiesis Simplified: Part 1 Erythropoiesis Larry Johnson Texas A&M University

2. Hematopoiesis Simplified: Part 1 Erythropoiesis Objectives are to: • Identify the developmental cells of erythropoiesis • Characterize developmental changes in the cytoplasm and nucleus of these cells Bone marrow 32583 Blood vessel

3. 19761 Bone marrow Blood in vessel

4. Peripheral blood 21 Erythrocytes Reticulocytes

5. Erythropoiesis Erythrocytes No nucleus and red cytoplasm Reticulocytes No nucleus and blue - grey cytoplasm Dark, small, spherical nucleus blue – grey cytoplasm Orthochromatophilic erythroblasts Darkening, fractured ,spherical nucleus and mixed pools of grey and blue cytoplasm Polychromatophilic erythroblasts Fractured, spherical nucleus and thin rim of dark blue cytoplasm Basophilic erythroblasts Uniformly light, spherical nucleus with thin rim of medium to dark blue cytoplasm Proerythroblasts

6. McGraw-Hill Companies

7. Erythropoiesis Proerythroblasts Erythrocytes Reticulocytes Orthochromatophilic erythroblasts Basophilic erythroblasts Polychromatophilic erythroblasts Erythropoiesis

8. Orthochromatophilic erythroblasts Erythrocytes

9. Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

10. Polychromatophilic erythroblasts

11. Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

12. Basophilic erythroblast

13. Erythrocyte Proerythroblast Reticulocytes Orthochromatophilic erythroblast Basophilic erythroblast Polychromatophilic erythroblast Erythropoiesis

14. Proerythroblasts

15. Erythropoiesis Orthochromatophilic erythroblasts Erythrocytes Reticulocytes

16. Earlyorthochromatophilic erythroblasts Latepolychromatophilic erythroblasts

17. Polychromatophilic erythroblasts Earlypolychromatophilic erythroblasts

18. Basophilic erythroblasts Proerythroblasts

19. ut 112 Orthochromatophilic erythroblasts RBCs Latepolychromatophilic erythroblast Basophilic erythroblast platelets Polychromatophilic erythroblast Reticulocyte Erythrocytes Eosinophil or basophil Proerythroblast

20. Bone marrow Reticulocyte entering blood vessel Blood vessel 32583

21. The End of Hematopoiesis Simplified: Part 1 Erythropoiesis

22. Granulopoiesis is next

23. Hematopoiesis Simplified: Part 2 Granulopoiesis Larry Johnson Texas A&M University

24. Hematopoiesis Simplified: Part 2 Granulopoiesis Objectives are to: • Identify the developmental cells of granulopoiesis • Characterize developmental changes in the cytoplasm and nucleus of these cells Bone marrow 32583

25. 19761 Bone marrow Blood Blood

26. ` 32583 Bone marrow Developing granulocytes

27. Peripheral blood 21

28. Granulopoiesis Lobulated, dark nucleus, mature grey cytoplasm with small granules Neutrophils Neutrophilic band or stab cells Horse-shoe shaped, darkening nucleus, grey mature cytoplasm Neutrophilic metamyelocytes Oval to kidney bean-shaped, indented nucleus, grey mature cytoplasm with a weak blue tent Large, oval, non-indented nucleus, large amount of cytoplasm with specific and non-specific granules Neutrophilic myelocytes Large, oval, non-indented nucleus, large amount of sky blue cytoplasm with non-specific granules Promyelocytes

30. Granulopoiesis Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilicmetamyelocytes

31. ut 112 Neutrophil Neutrophilic metamyelocytes Neutrophilic metamyelocyte Platelets Eosinophil or basophil Neutrophilic myelocytes

32. Neutrophils Promyelocytes Neutrophilic band cells Neutrophilic myelocytes Neutrophilicmetamyelocytes Granulopoiesis

33. Neutrophilicmetamyelocytes Lymphocytes Neutrophilicmyelocytes Unknown nuclei Promyelocyte Neutrophilic band cells Lymphocytes Neutrophilic myelocytes Neutrophilic band cells Neutrophilic metamyelocytes Neutrophilic band cell

34. Neutrophilicband cells Neutrophils Granulopoiesis

35. Neutrophilic metamyelocytes Earlyneutrophilic metamyelocytes

36. Neutrophilicmyelocytes

37. Latepromyelocytes Promyelocyte

38. Neutrophilic metamyelocytes Unknown nuclei Neutrophilic myelocytes Neutrophilic band cells Neutrophilic metamyelocytes Proerythroblast Orthochromatophilic erythroblasts Orthochromatophilic erythroblasts Eosinophils or basophils

39. Bone marrow 32583 White blood cell entering a blood vessel Blood vessel Blood vessel

40. The End of Hematopoiesis Simplified: Part 2 Granulopoiesis